Susceptibility-weighted imaging for stem cell visualization in a rat photothrombotic cerebral infarction model
DC Field | Value | Language |
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dc.contributor.author | Ha, Bon Chul | - |
dc.contributor.author | Jung, Jisung | - |
dc.contributor.author | Kwak, Byung Kook | - |
dc.date.available | 2019-03-08T17:58:33Z | - |
dc.date.issued | 2015-02 | - |
dc.identifier.issn | 0284-1851 | - |
dc.identifier.issn | 1600-0455 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/9897 | - |
dc.description.abstract | Background: In cell therapy, magnetic resonance imaging (MRI) has been used to visualize superparamagnetic iron oxide (SPIO)-labeled stem cells homing to a lesion. Improving traceability is to utilize the sequence that maximizes sensitivity to the susceptibility effect of SPIO. Purpose: To explore the best method by comparing the MRI sequences to visualize mesenchymal stem cells (MSCs) labeled with SPIO. Material and Methods: Human bone marrow (hBM)-derived MSCs were labeled by internalization of SPIO nanoparticles. In vitro MRI was performed for the SPIO-labeled hBM-MSCs in tubes with T2-weighted (T2W), T2*-weighted (T2*W), and susceptibility-weighted images (SWI). Contrast-to-noise ratio (CNR) and volumes of dark signal of SPIO-labeled hBM-MSCs were obtained on images of each sequence. Photothrombotic cerebral infarction (PTCI) was induced in eight rats, and 2.5 x 10(5) SPIO-labeled hBM-MSCs were infused through the tail vein on the third day. In vivo MRI of the rat brain was performed using a 3.0 T MRI on the first, third, seventh, and 14th days. CNRspio was obtained on T2W imaging, T2*W imaging, and SWI. The dark signals were compared with the SPIO-positive cells of Prussian blue staining. Results: In vitro MRI of 5 x 10(5) SPIO-labeled hBM-MSCs showed the CNR and volume of dark signal to be 63, 517 mm(3) in SWI, 41, 228 mm(3) in T2*W imaging, and 56, 41 mm(3) in T2W imaging, respectively. In vivo MRI showed a dark signal surrounding the high signal intensity of PTCI. Pathologically, the dark signals were matched with SPIO-labeled hBM-MSC in the corresponding rat. The dark signal was most prominent in SWI, then T2*W imaging, and finally in T2W imaging (P <0.05). In SWI, other causes of dark signals were matched with the veins and the choroid plexuses on histopathology. Conclusion: SWI can visualize SPIO-labeled hBM-MSCs more sensitively, earlier, and with larger size and greater contrast than T2W imaging and T2*W imaging. | - |
dc.format.extent | 9 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | SAGE PUBLICATIONS LTD | - |
dc.title | Susceptibility-weighted imaging for stem cell visualization in a rat photothrombotic cerebral infarction model | - |
dc.type | Article | - |
dc.identifier.doi | 10.1177/0284185114525605 | - |
dc.identifier.bibliographicCitation | ACTA RADIOLOGICA, v.56, no.2, pp 219 - 227 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000348141000013 | - |
dc.identifier.scopusid | 2-s2.0-84925581883 | - |
dc.citation.endPage | 227 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 219 | - |
dc.citation.title | ACTA RADIOLOGICA | - |
dc.citation.volume | 56 | - |
dc.type.docType | Article | - |
dc.publisher.location | 영국 | - |
dc.subject.keywordAuthor | Susceptibility-weighted imaging (SWI) | - |
dc.subject.keywordAuthor | magnetic resonance imaging (MRI) | - |
dc.subject.keywordAuthor | superparamagnetic iron oxide (SPIO) | - |
dc.subject.keywordAuthor | stem cell | - |
dc.subject.keywordAuthor | photothrombotic cerebral infarction | - |
dc.subject.keywordAuthor | rat | - |
dc.subject.keywordPlus | SUPERPARAMAGNETIC IRON-OXIDE | - |
dc.subject.keywordPlus | CLINICAL-APPLICATIONS | - |
dc.subject.keywordPlus | TRACKING | - |
dc.subject.keywordPlus | MRI | - |
dc.relation.journalResearchArea | Radiology, Nuclear Medicine & Medical Imaging | - |
dc.relation.journalWebOfScienceCategory | Radiology, Nuclear Medicine & Medical Imaging | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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