DA-9801 Promotes Neurite Outgrowth via ERK1/2-CREB Pathway in PC12 Cells
DC Field | Value | Language |
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dc.contributor.author | Won, Jong Hoon | - |
dc.contributor.author | Ahn, Kyong Hoon | - |
dc.contributor.author | Back, Moon Jung | - |
dc.contributor.author | Ha, Hae Chan | - |
dc.contributor.author | Jang, Ji Min | - |
dc.contributor.author | Kim, Ha Hyung | - |
dc.contributor.author | Choi, Sang-Zin | - |
dc.contributor.author | Son, Miwon | - |
dc.contributor.author | Kim, Dae Kyong | - |
dc.date.available | 2019-03-08T17:58:51Z | - |
dc.date.issued | 2015-02 | - |
dc.identifier.issn | 0918-6158 | - |
dc.identifier.issn | 1347-5215 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/9912 | - |
dc.description.abstract | In the present study, we examined the mechanisms underlying the effect of DA-9801 on neurite outgrowth. We found that DA-9801 elicits its effects via the mitogen-activated protein kinase (MEK) extracellular signal-regulated kinase (ERK)1/2-cAMP response element-binding protein (CREB) pathway. DA-9801, an extract from a mixture of Dioscorea japonica and Dioscorea nipponica, was reported to promote neurite outgrowth in PC12 cells. The effects of DA-9801 on cell viability and expression of neuronal markers were evaluated in PC12 cells. To investigate DA-9801 action, specific inhibitors targeting the ERK signaling cascade were used. No cytotoxicity was observed in PC12 cells at DA-9801 concentrations of less than 30 mu g/mL. In the presence of nerve growth factor (NGF, 2 ng/mL), DA-9801 promoted neurite outgrowth and increased the relative mRNA levels of neurofilament-L (NF-L), a marker of neuronal differentiation. The Raf-1 inhibitor GW5074 and MEK inhibitor PD98059 significantly attenuated DA-9801-induced neurite outgrowth. Additionally, the MEK1 and MEK2 inhibitor SL327 significantly attenuated the increase in the percentage of neurite-bearing PC12 cells induced by DA-9801 treatment. Conversely, the selective p38 mitogen-activated protein kinase inhibitor SB203580 did not attenuate the DA-9801 treatment-induced increase in the percentage of neurite-bearing PC12 cells. DA-9801 enhanced the phosphorylation of ERK1/2 and CREB in PC12 cells incubated with and without NGF. Pretreatment with PD98059 blocked the DA-9801-induced phosphorylation of ERK1/2 and CREB. In conclusion, DA-9801 induces neurite outgrowth by affecting the ERK1/2-CREB signaling pathway. Insights into the mechanism underlying this effect of DA-9801 may suggest novel potential strategies for the treatment of peripheral neuropathy. | - |
dc.format.extent | 10 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | PHARMACEUTICAL SOC JAPAN | - |
dc.title | DA-9801 Promotes Neurite Outgrowth via ERK1/2-CREB Pathway in PC12 Cells | - |
dc.type | Article | - |
dc.identifier.doi | 10.1248/bpb.b14-00236 | - |
dc.identifier.bibliographicCitation | BIOLOGICAL & PHARMACEUTICAL BULLETIN, v.38, no.2, pp 169 - 178 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000348690200002 | - |
dc.identifier.scopusid | 2-s2.0-84964199498 | - |
dc.citation.endPage | 178 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 169 | - |
dc.citation.title | BIOLOGICAL & PHARMACEUTICAL BULLETIN | - |
dc.citation.volume | 38 | - |
dc.type.docType | Article | - |
dc.publisher.location | 일본 | - |
dc.subject.keywordAuthor | DA-9801 | - |
dc.subject.keywordAuthor | diabetic peripheral neuropathy | - |
dc.subject.keywordAuthor | neurite outgrowth | - |
dc.subject.keywordAuthor | extracellular signal-regulated kinase 1/2 | - |
dc.subject.keywordAuthor | cAMP response element-binding protein (CREB) | - |
dc.subject.keywordPlus | NERVE GROWTH-FACTOR | - |
dc.subject.keywordPlus | ACTIVATED PROTEIN-KINASE | - |
dc.subject.keywordPlus | NEUROPATHIC PAIN | - |
dc.subject.keywordPlus | B-RAF | - |
dc.subject.keywordPlus | SIGNALING PATHWAYS | - |
dc.subject.keywordPlus | DIABETIC-RATS | - |
dc.subject.keywordPlus | MAP KINASE | - |
dc.subject.keywordPlus | PHARMACOLOGICAL MANAGEMENT | - |
dc.subject.keywordPlus | NEURONAL DIFFERENTIATION | - |
dc.subject.keywordPlus | NEUROTROPHIN RECEPTORS | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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