Unraveling the Novel Structure and Biosynthetic Pathway of O-Linked Glycans in the Golgi Apparatus of the Human Pathogenic Yeast Cryptococcus neoformans
DC Field | Value | Language |
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dc.contributor.author | Lee, Dong-Jik | - |
dc.contributor.author | Bahn, Yong-Sun | - |
dc.contributor.author | Kim, Hong-Jin | - |
dc.contributor.author | Chung, Seung-Yeon | - |
dc.contributor.author | Kang, Hyun Ah | - |
dc.date.available | 2019-03-08T18:00:05Z | - |
dc.date.issued | 2015-01 | - |
dc.identifier.issn | 0021-9258 | - |
dc.identifier.issn | 1083-351X | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/9968 | - |
dc.description.abstract | Cryptococcus neoformans is an encapsulated basidiomycete causing cryptococcosis in immunocompromised humans. The cell surface mannoproteins of C. neoformans were reported to stimulate the host T-cell response and to be involved in fungal pathogenicity; however, their O-glycan structure is uncharacterized. In this study, we performed a detailed structural analysis of the O-glycans attached to cryptococcal mannoproteins using HPLC combined with exoglycosidase treatment and showed that the major C. neoformans O-glycans were short manno-oligosaccharides that were connected mostly by alpha 1,2-linkages but connected by an alpha 1,6-linkage at the third mannose residue. Comparison of the O-glycan profiles from wild-type and uxs1 Delta mutant strains strongly supports the presence of minor O-glycans carrying a xylose residue. Further analyses of C. neoformans mutant strains identified three mannosyltransferase genes involved in O-glycan extensions in the Golgi. C. neoformans KTR3, the only homolog of the Saccharomyces cerevisiae KRE2/MNT1 family genes, was shown to encode an alpha 1,2-mannosyltransferase responsible for the addition of the second mannose residue via an alpha 1,2-linkage to the major O-glycans. C. neoformans HOC1 and HOC3, homologs of the Saccharomyces cerevisiae OCH1 family genes, were shown to encode alpha 1,6-mannosyltransferases that can transfer the third mannose residue, via an alpha 1,6-linkage, to minor O-glycans containing xylose and to major O-glycans without xylose, respectively. Moreover, the C. neoformans ktr3 Delta mutant strain, which displayed increased sensitivity to SDS, high salt, and high temperature, showed attenuated virulence in a mouse model of cryptococcosis, suggesting that the extended structure of O-glycans is required for cell integrity and full pathogenicity of C. neoformans. | - |
dc.format.extent | 13 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC | - |
dc.title | Unraveling the Novel Structure and Biosynthetic Pathway of O-Linked Glycans in the Golgi Apparatus of the Human Pathogenic Yeast Cryptococcus neoformans | - |
dc.type | Article | - |
dc.identifier.doi | 10.1074/jbc.M114.607705 | - |
dc.identifier.bibliographicCitation | JOURNAL OF BIOLOGICAL CHEMISTRY, v.290, no.3, pp 1861 - 1873 | - |
dc.description.isOpenAccess | Y | - |
dc.identifier.wosid | 000348056400050 | - |
dc.identifier.scopusid | 2-s2.0-84921328271 | - |
dc.citation.endPage | 1873 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 1861 | - |
dc.citation.title | JOURNAL OF BIOLOGICAL CHEMISTRY | - |
dc.citation.volume | 290 | - |
dc.type.docType | Article | - |
dc.publisher.location | 미국 | - |
dc.subject.keywordPlus | CELL-WALL INTEGRITY | - |
dc.subject.keywordPlus | SACCHAROMYCES-CEREVISIAE | - |
dc.subject.keywordPlus | CANDIDA-ALBICANS | - |
dc.subject.keywordPlus | SCHIZOSACCHAROMYCES-POMBE | - |
dc.subject.keywordPlus | PICHIA-PASTORIS | - |
dc.subject.keywordPlus | FUNCTIONAL-CHARACTERIZATION | - |
dc.subject.keywordPlus | MANNOSYL GLYCANS | - |
dc.subject.keywordPlus | GENE FAMILY | - |
dc.subject.keywordPlus | GLYCOSYLATION | - |
dc.subject.keywordPlus | MANNOSYLTRANSFERASE | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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