Switchable Self-Assembly of Elastin- and Resilin-Based Block Copolypeptides with Converse Phase Transition Behaviors
DC Field | Value | Language |
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dc.contributor.author | Basheer, Aamna | - |
dc.contributor.author | Shahid, Shahzaib | - |
dc.contributor.author | Kang, Min Jung | - |
dc.contributor.author | Lee, Jae Hee | - |
dc.contributor.author | Lee, Jae Sang | - |
dc.contributor.author | Lim, Dong Woo | - |
dc.date.accessioned | 2021-07-28T08:08:03Z | - |
dc.date.available | 2021-07-28T08:08:03Z | - |
dc.date.issued | 2021-06 | - |
dc.identifier.issn | 1944-8244 | - |
dc.identifier.issn | 1944-8252 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/105731 | - |
dc.description.abstract | Self-assembly of thermally responsive polypeptides into unique nanostructures offers intriguing attributes including dynamic physical dimensions, biocompatibility, and biodegradability for the smart bio-nanomaterials. As elastin-based polypeptide (EBP) fusion proteins with lower critical solution temperature (LCST) are studied as drug delivery systems, EBP block copolypeptides with the resilin-based polypeptide (RBP) displaying an upper critical solution temperature (UCST) have been of great interest. In this study, we report thermally triggered, dynamic self-assembly of EBP- and RBP-based diblock copolypeptides into switched nanostructures with reversibility under physiological conditions. Molecular DNA clones encoding for the EBP-RBP diblocks at different block length ratios were biosynthesized via recursive directional ligation and overexpressed, followed by nonchromatographic purification by inverse transition cycling. Genetically engineered diblock copolypeptides composed of the EBP with an LCST and the RBP with a UCST showed converse phase transition behaviors with both a distinct LCST and a distinct UCST (LCST < UCST). As temperature increased, three phases of these EBP-RBP diblocks were observed: (1) self-assembled micelles or vesicles below both LCST and UCST, (2) whole aggregates above LCST and below UCST, and (3) reversed micelles above both LCST and UCST. In conclusion, these stimuli-triggered, dynamic protein-based nanostructures are promising for advanced drug delivery systems, regenerative medicine, and biomedical nanotechnology. | - |
dc.format.extent | 16 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.title | Switchable Self-Assembly of Elastin- and Resilin-Based Block Copolypeptides with Converse Phase Transition Behaviors | - |
dc.type | Article | - |
dc.publisher.location | 미국 | - |
dc.identifier.doi | 10.1021/acsami.1c00676 | - |
dc.identifier.scopusid | 2-s2.0-85107710815 | - |
dc.identifier.wosid | 000659315800004 | - |
dc.identifier.bibliographicCitation | ACS APPLIED MATERIALS & INTERFACES, v.13, no.21, pp 24385 - 24400 | - |
dc.citation.title | ACS APPLIED MATERIALS & INTERFACES | - |
dc.citation.volume | 13 | - |
dc.citation.number | 21 | - |
dc.citation.startPage | 24385 | - |
dc.citation.endPage | 24400 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalResearchArea | Materials Science | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Multidisciplinary | - |
dc.subject.keywordPlus | RESPONSIVE POLYMERIC NANOPARTICLES | - |
dc.subject.keywordPlus | RECURSIVE DIRECTIONAL LIGATION | - |
dc.subject.keywordPlus | PROTEIN-BASED POLYMERS | - |
dc.subject.keywordPlus | COPOLYMER MICELLES | - |
dc.subject.keywordPlus | THERMORESPONSIVE POLYMERS | - |
dc.subject.keywordPlus | DRUG-DELIVERY | - |
dc.subject.keywordPlus | POLYPEPTIDE | - |
dc.subject.keywordPlus | TEMPERATURE | - |
dc.subject.keywordPlus | UCST | - |
dc.subject.keywordPlus | PURIFICATION | - |
dc.subject.keywordAuthor | switchable self-assembly | - |
dc.subject.keywordAuthor | elastin | - |
dc.subject.keywordAuthor | resilin | - |
dc.subject.keywordAuthor | block copolypeptides | - |
dc.subject.keywordAuthor | converse phase transition behaviors | - |
dc.identifier.url | https://pubs.acs.org/doi/10.1021/acsami.1c00676 | - |
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