Simultaneous detection of SARS-CoV-2 and identification of spike D614G mutation using point-of-care real-time polymerase chain reaction
DC Field | Value | Language |
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dc.contributor.author | Lee, So Yul | - |
dc.contributor.author | Lee, Ji Su | - |
dc.contributor.author | Ahn, Jeong Jin | - |
dc.contributor.author | Kim, Seung Jun | - |
dc.contributor.author | Sung, Heungsup | - |
dc.contributor.author | Huh, Jin Won | - |
dc.contributor.author | Hwang, Seung Yong | - |
dc.date.accessioned | 2022-07-06T02:47:24Z | - |
dc.date.available | 2022-07-06T02:47:24Z | - |
dc.date.created | 2022-06-08 | - |
dc.date.issued | 2022-06 | - |
dc.identifier.issn | 0166-0934 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/107690 | - |
dc.description.abstract | Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is associated with high mortality and infectivity rates in humans since its emergence. Analysis using high-accuracy real-time polymerase chain reaction (PCR) is recommended for the detection of general respiratory viruses including SARS-CoV-2, but it takes a long time (e.g. ~ 6 h); moreover, on-site diagnosis is difficult owing to the need for skilled technicians and advanced laboratory facilities. Currently, the importance of point-of-care testing (POCT) is being emphasized for the rapid detection of SARS-CoV-2. Here, we developed a multiplex real-time reverse transcription PCR (rRT-PCR) analysis that not only detects SARS-CoV-2 but also D614G strains with higher contagiousness than wild types among SARS-CoV-2 mutants using probe-based rRT-PCR. Moreover, this method was applied to portable PCR equipment capable of POCT to confirm high detection sensitivity and specificity. Multiple assays were possible with fluorescence labeling of individual probes. Furthermore, using a microfluidic chip-based point-of-care testing rRT-PCR platform, detection time was reduced by more than half compared with the commonly used detection system. This demonstrates that our assay has 100% of high sensitivity and specificity and could thus aid in the rapid and simple screening of SARS-CoV-2 carrying the mutation. We present a rapid detection method for mutations in SARS-CoV-2. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | Elsevier BV | - |
dc.title | Simultaneous detection of SARS-CoV-2 and identification of spike D614G mutation using point-of-care real-time polymerase chain reaction | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Hwang, Seung Yong | - |
dc.identifier.doi | 10.1016/j.jviromet.2022.114513 | - |
dc.identifier.scopusid | 2-s2.0-85125876897 | - |
dc.identifier.wosid | 000795589800009 | - |
dc.identifier.bibliographicCitation | Journal of Virological Methods, v.304, pp.1 - 4 | - |
dc.relation.isPartOf | Journal of Virological Methods | - |
dc.citation.title | Journal of Virological Methods | - |
dc.citation.volume | 304 | - |
dc.citation.startPage | 1 | - |
dc.citation.endPage | 4 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Virology | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Virology | - |
dc.subject.keywordAuthor | SARS-CoV-2 | - |
dc.subject.keywordAuthor | Spike D614G mutation | - |
dc.subject.keywordAuthor | Point-of-care | - |
dc.subject.keywordAuthor | Real-time PCR | - |
dc.subject.keywordAuthor | COVID-19 | - |
dc.subject.keywordAuthor | Molecular diagnosis | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S016609342200060X?via%3Dihub | - |
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