Glyco-Steroidal Amphiphiles (GSAs) for Membrane Protein Structural Study
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Ehsan, Muhammad | - |
dc.contributor.author | Wang, Haoqing | - |
dc.contributor.author | Katsube, Satoshi | - |
dc.contributor.author | Munk, Chastine F. | - |
dc.contributor.author | Du, Yang | - |
dc.contributor.author | Youn, Taeyeol | - |
dc.contributor.author | Yoon, Soyoung | - |
dc.contributor.author | Byrne, Bernadette | - |
dc.contributor.author | Loland, Claus J. | - |
dc.contributor.author | Guan, Lan | - |
dc.contributor.author | Kobilka, Brian K. | - |
dc.contributor.author | Chae, Pil Seok | - |
dc.date.accessioned | 2022-07-18T01:18:08Z | - |
dc.date.available | 2022-07-18T01:18:08Z | - |
dc.date.issued | 2022-04 | - |
dc.identifier.issn | 1439-4227 | - |
dc.identifier.issn | 1439-7633 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/107924 | - |
dc.description.abstract | Integral membrane proteins pose considerable challenges to high resolution structural analysis. Maintaining membrane proteins in their native state during protein isolation is essential for structural study of these bio-macromolecules. Detergents are the most commonly used amphiphilic compounds for stabilizing membrane proteins in solution outside a lipid bilayer. We previously introduced a glyco-diosgenin (GDN) detergent that was shown to be highly effective at stabilizing a wide range of membrane proteins. This steroidal detergent has additionally gained attention due to its compatibility with membrane protein structure study via cryo-EM. However, synthetic inconvenience limits widespread use of GDN in membrane protein study. To improve its synthetic accessibility and to further enhance detergent efficacy for protein stabilization, we designed a new class of glyco-steroid-based detergents using three steroid units: cholestanol, cholesterol and diosgenin. These new detergents were efficiently prepared and showed marked efficacy for protein stabilization in evaluation with a few model membrane proteins including two G protein-coupled receptors. Some new agents were not only superior to a gold standard detergent, DDM (n-dodecyl-beta-d-maltoside), but were also more effective than the original GDN at preserving protein integrity long term. These agents represent valuable alternatives to GDN, and are likely to facilitate structural determination of challenging membrane proteins. | - |
dc.format.extent | 8 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | John Wiley & Sons Ltd. | - |
dc.title | Glyco-Steroidal Amphiphiles (GSAs) for Membrane Protein Structural Study | - |
dc.type | Article | - |
dc.publisher.location | 독일 | - |
dc.identifier.doi | 10.1002/cbic.202200027 | - |
dc.identifier.scopusid | 2-s2.0-85124910064 | - |
dc.identifier.wosid | 000758363700001 | - |
dc.identifier.bibliographicCitation | ChemBioChem, v.23, no.7, pp 1 - 8 | - |
dc.citation.title | ChemBioChem | - |
dc.citation.volume | 23 | - |
dc.citation.number | 7 | - |
dc.citation.startPage | 1 | - |
dc.citation.endPage | 8 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Medicinal | - |
dc.subject.keywordPlus | CRYSTAL-STRUCTURE | - |
dc.subject.keywordPlus | DETERGENT | - |
dc.subject.keywordPlus | CHOLESTEROL | - |
dc.subject.keywordPlus | RECEPTOR | - |
dc.subject.keywordPlus | CRYSTALLIZATION | - |
dc.subject.keywordPlus | SOLUBILIZATION | - |
dc.subject.keywordPlus | STABILIZATION | - |
dc.subject.keywordPlus | FLUORESCENCE | - |
dc.subject.keywordPlus | NANODISCS | - |
dc.subject.keywordPlus | TRANSPORT | - |
dc.subject.keywordAuthor | detergent design | - |
dc.subject.keywordAuthor | detergent-detergent interactions | - |
dc.subject.keywordAuthor | glyco-steroids | - |
dc.subject.keywordAuthor | glycolipids | - |
dc.subject.keywordAuthor | protein stabilization | - |
dc.identifier.url | https://www.scopus.com/record/display.uri?eid=2-s2.0-85124910064&origin=inward&txGid=96cc36cf7030f0b5feaab5a541a1959b | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
55 Hanyangdeahak-ro, Sangnok-gu, Ansan, Gyeonggi-do, 15588, Korea+82-31-400-4269 sweetbrain@hanyang.ac.kr
COPYRIGHT © 2021 HANYANG UNIVERSITY. ALL RIGHTS RESERVED.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.