Extracellular vesicles from adipose tissue-derived stem cells alleviate osteoporosis through osteoprotegerin and miR-21-5p
DC Field | Value | Language |
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dc.contributor.author | Lee, Kyoung Soo | - |
dc.contributor.author | Lee, Jeongmi | - |
dc.contributor.author | Kim, Hark Kyun | - |
dc.contributor.author | Yeom, Seung Ho | - |
dc.contributor.author | Woo, Chang Hee | - |
dc.contributor.author | Jung, Youn Jae | - |
dc.contributor.author | Yun, Ye Eun | - |
dc.contributor.author | Park, So Young | - |
dc.contributor.author | Han, Jihoon | - |
dc.contributor.author | Kim, Eunae | - |
dc.contributor.author | Sul, Jae Hoon | - |
dc.contributor.author | Jung, Jae Min | - |
dc.contributor.author | Park, Jae Hyung | - |
dc.contributor.author | Choi, Ji Suk | - |
dc.contributor.author | Cho, Yong Woo | - |
dc.contributor.author | Jo, Dong-Gyu | - |
dc.date.accessioned | 2022-07-18T01:32:01Z | - |
dc.date.available | 2022-07-18T01:32:01Z | - |
dc.date.issued | 2021-10 | - |
dc.identifier.issn | 2001-3078 | - |
dc.identifier.issn | 2001-3078 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/108195 | - |
dc.description.abstract | Osteoporosis is one of the most common skeletal disorders caused by the imbalance between bone formation and resorption, resulting in quantitative loss of bone tissue. Since stem cell-derived extracellular vesicles (EVs) are growing attention as novel cell-free therapeutics that have advantages over parental stem cells, the therapeutic effects of EVs from adipose tissue-derived stem cells (ASGEVs) on osteoporosis pathogenesis were investigated. ASC-EVs were isolated by a multi-filtration system based on the tangential flow filtration (TFF) system and characterized using transmission electron microscopy, dynamic light scattering, zeta potential, flow cytometry, cytokine arrays, and enzyme-linked immunosorbent assay. EVs are rich in growth factors and cytokines related to bone metabolism and mesenchymal stem cell (MSC) migration. In particular, osteoprotegerin (OPG), a natural inhibitor of receptor activator of nuclear factor-xB ligand (RANKL), was highly enriched in ASCEVs. We found that the intravenous administration of ASC-EVs attenuated bone loss in osteoporosis mice. Also, ASC-EVs significantly inhibited osteoclast differentiation of macrophages and promoted the migration of bone marrow-derived MSCs (BM-MSCs). However, OPG-depleted ASC-EVs did not show anti-osteoclastogenesis effects, demonstrating that OPG is critical for the therapeutic effects of ASC-EVs. Additionally, small RNA sequencing data were analysed to identify miRNA candidates related to anti-osteoporosis effects. miR-21-5p in ASG-EVs inhibited osteoclast differentiation through Acvr2a down-regulation. Also, let-7b-5p in ASC-EVs significantly reduced the expression of genes related to osteoclastogenesis. Finally, ASC-EVs reached the bone tissue after they were injected intravenously, and they remained longer. OPG, miR-21-5p, and let-7b-5p in ASC-EVs inhibit osteoclast differentiation and reduce gene expression related to bone resorption, suggesting that ASC-EVs are highly promising as cell-free therapeutic agents for osteoporosis treatment. | - |
dc.format.extent | 18 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | WILEY | - |
dc.title | Extracellular vesicles from adipose tissue-derived stem cells alleviate osteoporosis through osteoprotegerin and miR-21-5p | - |
dc.type | Article | - |
dc.publisher.location | 미국 | - |
dc.identifier.doi | 10.1002/jev2.12152 | - |
dc.identifier.scopusid | 2-s2.0-85117396354 | - |
dc.identifier.wosid | 000702295400001 | - |
dc.identifier.bibliographicCitation | JOURNAL OF EXTRACELLULAR VESICLES, v.10, no.12, pp 1 - 18 | - |
dc.citation.title | JOURNAL OF EXTRACELLULAR VESICLES | - |
dc.citation.volume | 10 | - |
dc.citation.number | 12 | - |
dc.citation.startPage | 1 | - |
dc.citation.endPage | 18 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.subject.keywordPlus | BONE-MARROW | - |
dc.subject.keywordPlus | EXOSOMES | - |
dc.subject.keywordPlus | DIFFERENTIATION | - |
dc.subject.keywordPlus | OSTEOGENESIS | - |
dc.subject.keywordPlus | THERAPY | - |
dc.subject.keywordPlus | GENE | - |
dc.subject.keywordPlus | MASS | - |
dc.subject.keywordAuthor | extracellular vesicles | - |
dc.subject.keywordAuthor | human adipose tissue-derived stem cells | - |
dc.subject.keywordAuthor | let-7b-5p | - |
dc.subject.keywordAuthor | miR-21-5p | - |
dc.subject.keywordAuthor | osteoporosis | - |
dc.subject.keywordAuthor | osteoprotegerin | - |
dc.identifier.url | https://onlinelibrary.wiley.com/doi/10.1002/jev2.12152 | - |
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