3,4-Bis(hydroxymethyl)hexane-1,6-diol-based Maltosides (HDMs) for Membrane-Protein Study: Importance of Detergent Rigidity-Flexibility Balance in Protein Stability
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Hyun Sung | - |
dc.contributor.author | Das, Manabendra | - |
dc.contributor.author | Mahler, Florian | - |
dc.contributor.author | Ahmed, Waqar | - |
dc.contributor.author | Wang, Haoqing | - |
dc.contributor.author | Mortensen, Jonas S. | - |
dc.contributor.author | Hariharan, Parameswaran | - |
dc.contributor.author | Ghani, Lubna | - |
dc.contributor.author | Byrne, Bernadette | - |
dc.contributor.author | Guan, Lan | - |
dc.contributor.author | Loland, Claus J. | - |
dc.contributor.author | Keller, Sandro | - |
dc.contributor.author | Chae, Pil Seok | - |
dc.date.accessioned | 2022-12-20T04:36:16Z | - |
dc.date.available | 2022-12-20T04:36:16Z | - |
dc.date.issued | 2022-12 | - |
dc.identifier.issn | 1861-4728 | - |
dc.identifier.issn | 1861-471X | - |
dc.identifier.uri | https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/111188 | - |
dc.description.abstract | Detergents have been major contributors to membrane-protein structural study for decades. However, membrane proteins solubilized in conventional detergents tend to aggregate or denature over time. Stability of large eukaryotic membrane proteins with complex structures tends to be particularly poor, necessitating development of novel detergents with improved properties. Here, we prepared a novel class of detergents, designated 3,4-bis(hydroxymethyl)hexane-1,6-diol-based maltosides (HDMs). When tested on three membrane proteins, including two G-protein-coupled receptors (GPCRs), the new detergents displayed significantly better behaviors compared with DDM. Moreover, the HDMs were superior or comparable to LMNG, an amphiphile widely used for GPCR structural study. An optimal balance of detergent rigidity vs. flexibility of the HDMs is likely responsible for their favorable behaviors toward membrane-protein stability. Thus, the current study not only introduces the HDMs, with significant potential for membrane-protein structural study, but also suggests a useful guideline for designing novel detergents for membrane-protein research. | - |
dc.format.extent | 8 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Wiley - V C H Verlag GmbbH & Co. | - |
dc.title | 3,4-Bis(hydroxymethyl)hexane-1,6-diol-based Maltosides (HDMs) for Membrane-Protein Study: Importance of Detergent Rigidity-Flexibility Balance in Protein Stability | - |
dc.type | Article | - |
dc.publisher.location | 독일 | - |
dc.identifier.doi | 10.1002/asia.202200941 | - |
dc.identifier.scopusid | 2-s2.0-85141589019 | - |
dc.identifier.wosid | 000879919500001 | - |
dc.identifier.bibliographicCitation | Chemistry - An Asian Journal, v.17, no.24, pp 1 - 8 | - |
dc.citation.title | Chemistry - An Asian Journal | - |
dc.citation.volume | 17 | - |
dc.citation.number | 24 | - |
dc.citation.startPage | 1 | - |
dc.citation.endPage | 8 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.subject.keywordPlus | BETA(2)-ADRENERGIC RECEPTOR | - |
dc.subject.keywordPlus | FACIAL AMPHIPHILES | - |
dc.subject.keywordPlus | CRYSTAL-STRUCTURE | - |
dc.subject.keywordPlus | GNG AMPHIPHILES | - |
dc.subject.keywordPlus | STABILIZATION | - |
dc.subject.keywordPlus | CRYSTALLIZATION | - |
dc.subject.keywordPlus | SOLUBILIZATION | - |
dc.subject.keywordPlus | SURFACTANTS | - |
dc.subject.keywordPlus | SYSTEMS | - |
dc.subject.keywordPlus | YIELDS | - |
dc.subject.keywordAuthor | amphiphiles | - |
dc.subject.keywordAuthor | membrane proteins | - |
dc.subject.keywordAuthor | molecular design | - |
dc.subject.keywordAuthor | HDM | - |
dc.subject.keywordAuthor | molecular flexibility | - |
dc.identifier.url | https://onlinelibrary.wiley.com/doi/10.1002/asia.202200941 | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
55 Hanyangdeahak-ro, Sangnok-gu, Ansan, Gyeonggi-do, 15588, Korea+82-31-400-4269 sweetbrain@hanyang.ac.kr
COPYRIGHT © 2021 HANYANG UNIVERSITY. ALL RIGHTS RESERVED.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.