Protective Effect of Alpinia oxyphylla Fruit against tert-Butyl Hydroperoxide-Induced Toxicity in HepG2 Cells via Nrf2 Activation and Free Radical Scavenging and Its Active Molecules
DC Field | Value | Language |
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dc.contributor.author | Park, Chae Lee | - |
dc.contributor.author | Kim, Ji Hoon | - |
dc.contributor.author | Jeon, Je-Seung | - |
dc.contributor.author | Lee, Ju-hee | - |
dc.contributor.author | Zhang, Kaixuan | - |
dc.contributor.author | Guo, Shuo | - |
dc.contributor.author | Lee, Do-hyun | - |
dc.contributor.author | Gao, Eun Mei | - |
dc.contributor.author | Son, Rak Ho | - |
dc.contributor.author | Kim, Young-Mi | - |
dc.contributor.author | Park, Gyu Hwan | - |
dc.contributor.author | Kim, Chul Young | - |
dc.date.accessioned | 2022-12-20T05:50:25Z | - |
dc.date.available | 2022-12-20T05:50:25Z | - |
dc.date.issued | 2022-05 | - |
dc.identifier.issn | 2076-3921 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/111301 | - |
dc.description.abstract | Alpinia oxyphylla Miq. (Zingiberaceae) extract exerts protective activity against tert-butyl hydroperoxide-induced toxicity in HepG2 cells, and the antioxidant response element (ARE) luciferase activity increased 6-fold at 30 mu g/mL in HepG2 cells transiently transfected with ARE-luciferase. To identify active molecules, activity-guided isolation of the crude extract led to four sesquiterpenes (1, 2, 5, 6) and two diarylheptanoids (3 and 4) from an n-hexane extract and six sesquiterpenes (7-12) from an ethyl acetate extract. Chemical structures were elucidated by one-dimensional, two-dimensional nuclear magnetic resonance (1D-, 2D-NMR), and mass (MS) spectral data. Among the isolated compounds, eudesma-3,11-dien-2-one (2) promoted the nuclear accumulation of nuclear factor (erythroid-derived 2)-like 2 (Nrf2) and increased the promoter property of the ARE. Diarylheptanoids, yakuchinone A (3), and 5 '-hydroxyl-yakuchinone A (4) showed radical scavenging activity in 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 3-ethylbenzothiazoline-6-sulphonic acid (ABTS) assays. Furthermore, optimization of extraction solvents (ratios of water and ethanol) was performed by comparison of contents of active compounds, ARE-inducing activity, radical scavenging activity, and HepG2 cell protective activity. As a result, 75% ethanol was the best solvent for the extraction of A. oxyphylla fruit. This study demonstrated that A. oxyphylla exerted antioxidant effects via the Nrf2/HO-1 (heme oxygenase-1) pathway and radical scavenging along with active markers eudesma-3,11-dien-2-one (2) and yakuchinone A (3). | - |
dc.format.extent | 15 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | MDPI AG | - |
dc.title | Protective Effect of Alpinia oxyphylla Fruit against tert-Butyl Hydroperoxide-Induced Toxicity in HepG2 Cells via Nrf2 Activation and Free Radical Scavenging and Its Active Molecules | - |
dc.type | Article | - |
dc.publisher.location | 스위스 | - |
dc.identifier.doi | 10.3390/antiox11051032 | - |
dc.identifier.scopusid | 2-s2.0-85131864351 | - |
dc.identifier.wosid | 000801822600001 | - |
dc.identifier.bibliographicCitation | Antioxidants, v.11, no.5, pp 1 - 15 | - |
dc.citation.title | Antioxidants | - |
dc.citation.volume | 11 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 1 | - |
dc.citation.endPage | 15 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalResearchArea | Food Science & Technology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Medicinal | - |
dc.relation.journalWebOfScienceCategory | Food Science & Technology | - |
dc.subject.keywordPlus | DAMAGE IN-VITRO | - |
dc.subject.keywordPlus | OXIDATIVE DAMAGE | - |
dc.subject.keywordPlus | ETHANOL EXTRACT | - |
dc.subject.keywordPlus | ANTIOXIDANT | - |
dc.subject.keywordPlus | DIARYLHEPTANOIDS | - |
dc.subject.keywordPlus | SESQUITERPENES | - |
dc.subject.keywordPlus | ENZYMES | - |
dc.subject.keywordAuthor | Alpinia oxyphylla | - |
dc.subject.keywordAuthor | antioxidant activity | - |
dc.subject.keywordAuthor | eudesma-3 | - |
dc.subject.keywordAuthor | 11-dien-2-one | - |
dc.subject.keywordAuthor | yakuchinone A | - |
dc.identifier.url | https://www.mdpi.com/2076-3921/11/5/1032 | - |
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