Apta-sensor for selective determination of dopamine using chitosan-stabilized Prussian blue nanoparticles
DC Field | Value | Language |
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dc.contributor.author | Lee, Han Been | - |
dc.contributor.author | Son, Seong Eun | - |
dc.contributor.author | Seong, Gi Hun | - |
dc.date.accessioned | 2023-08-01T06:30:37Z | - |
dc.date.available | 2023-08-01T06:30:37Z | - |
dc.date.issued | 2023-08 | - |
dc.identifier.issn | 2050-7518 | - |
dc.identifier.issn | 2050-750X | - |
dc.identifier.uri | https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/113573 | - |
dc.description.abstract | Chitosan-stabilized Prussian blue nanoparticles (CS/PBNPs) were fabricated by a simple synthetic method and used to develop a novel aptamer-based colorimetric assay for selective determination of dopamine (DA). Scanning electron microscopy (SEM) images exhibited a uniform shape of the CS/PBNPs with an average diameter of 37.0 & PLUSMN; 3.2 nm. The CS/PBNPs exhibited strong peroxidase-like activity that catalyzed the reaction between 3,3 & PRIME;,5,5 & PRIME;-tetramethylbenzidine (TMB) and hydrogen peroxide (H2O2). Chitosan was used for stabilization of the PBNPs and fixation of the DA aptamer on the surface of the CS/PBNPs. The catalytic mechanism of the CS/PBNPs was confirmed to involve first the decomposition of H2O2 into a hydroxyl radical (OH) and then oxidation of TMB by the OH to produce a blue color. An aptamer-based colorimetric assay was made with the CS/PBNPs to detect DA at concentrations of 0.25-100 & mu;M with a limit of detection (LOD) of 0.16 & mu;M. For comparison, a gold nanoparticle (AuNP)-based apta-sensor detected DA in concentrations of 1-25 & mu;M with a LOD of 0.55 & mu;M. The recovery results of DA concentrations (0.25, 0.5, and 1 & mu;M) from spiked human serum were 92.6%, 102.1%, and 103.9%, verifying the reliability and reproducibility of the CS/PBNP-based apta-sensor for determination of DA level in clinical applications. Moreover, compared to traditional immunoassay, this aptamer-based nanozyme activation/inhibition system needs no washing step, which is very useful to shorten the assay time and maintain high sensitivity. | - |
dc.format.extent | 11 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Royal Society of Chemistry | - |
dc.title | Apta-sensor for selective determination of dopamine using chitosan-stabilized Prussian blue nanoparticles | - |
dc.type | Article | - |
dc.publisher.location | 영국 | - |
dc.identifier.doi | 10.1039/d3tb00799e | - |
dc.identifier.scopusid | 2-s2.0-85165427270 | - |
dc.identifier.wosid | 001022492300001 | - |
dc.identifier.bibliographicCitation | Journal of Materials Chemistry B, v.11, no.30, pp 1 - 11 | - |
dc.citation.title | Journal of Materials Chemistry B | - |
dc.citation.volume | 11 | - |
dc.citation.number | 30 | - |
dc.citation.startPage | 1 | - |
dc.citation.endPage | 11 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Materials Science | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Biomaterials | - |
dc.subject.keywordPlus | PEROXIDASE-LIKE ACTIVITY | - |
dc.subject.keywordPlus | IN-VITRO SELECTION | - |
dc.subject.keywordPlus | COLORIMETRIC DETECTION | - |
dc.subject.keywordPlus | LABEL-FREE | - |
dc.subject.keywordPlus | ELECTROCHEMICAL DETECTION | - |
dc.subject.keywordPlus | MAGNETIC NANOPARTICLES | - |
dc.subject.keywordPlus | GRAPHENE | - |
dc.subject.keywordPlus | MIMICKING | - |
dc.subject.keywordPlus | NANOTUBES | - |
dc.subject.keywordPlus | PLATFORM | - |
dc.identifier.url | https://pubs.rsc.org/en/content/articlelanding/2023/TB/D3TB00799E | - |
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