Self-organized insulin-producing β-cells differentiated from human omentum-derived stem cells and their in vivo therapeutic potential
DC Field | Value | Language |
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dc.contributor.author | Jeong, Ji Hoon | - |
dc.contributor.author | Park, Ki Nam | - |
dc.contributor.author | Kim, Joo Hyun | - |
dc.contributor.author | Noh, KyungMu | - |
dc.contributor.author | Hur, Sung Sik | - |
dc.contributor.author | Kim, Yunhye | - |
dc.contributor.author | Hong, Moonju | - |
dc.contributor.author | Chung, Jun Chul | - |
dc.contributor.author | Park, Jae Hong | - |
dc.contributor.author | Lee, Jongsoon | - |
dc.contributor.author | Son, Young-Ik | - |
dc.contributor.author | Lee, Ju Hun | - |
dc.contributor.author | Kim, Sang-Heon | - |
dc.contributor.author | Hwang, Yongsung | - |
dc.date.accessioned | 2023-09-11T01:31:17Z | - |
dc.date.available | 2023-09-11T01:31:17Z | - |
dc.date.issued | 2023-08 | - |
dc.identifier.issn | 1226-4601 | - |
dc.identifier.issn | 2055-7124 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/115124 | - |
dc.description.abstract | Background: Human omentum-derived mesenchymal stem cells (hO-MSCs) possess great potential to differentiate into multiple lineages and have self-renewal capacity, allowing them to be utilized as patient-specific cell-based therapeutics. Although the use of various stem cell-derived β-cells has been proposed as a novel approach for treating diabetes mellitus, developing an efficient method to establish highly functional β-cells remains challenging. Methods: We aimed to develop a novel cell culture platform that utilizes a fibroblast growth factor 2 (FGF2)-immobilized matrix to regulate the adhesion and differentiation of hO-MSCs into insulin-producing β-cells via cell–matrix/cell–cell interactions. In our study, we evaluated the in vitro differentiation potential of hO-MSCs cultured on an FGF2-immobilized matrix and a round-bottom plate (RBP). Further, the in vivo therapeutic efficacy of the β-cells transplanted into kidney capsules was evaluated using animal models with streptozotocin (STZ)-induced diabetes. Results: Our findings demonstrated that cells cultured on an FGF2-immobilized matrix could self-organize into insulin-producing β-cell progenitors, as evident from the upregulation of pancreatic β-cell-specific markers (PDX-1, Insulin, and Glut-2). Moreover, we observed significant upregulation of heparan sulfate proteoglycan, gap junction proteins (Cx36 and Cx43), and cell adhesion molecules (E-cadherin and Ncam1) in cells cultured on the FGF2-immobilized matrix. In addition, in vivo transplantation of differentiated β-cells into animal models of STZ-induced diabetes revealed their survival and engraftment as well as glucose-sensitive production of insulin within the host microenvironment, at over 4 weeks after transplantation. Conclusions: Our findings suggest that the FGF2-immobilized matrix can support initial cell adhesion, maturation, and glucose-stimulated insulin secretion within the host microenvironment. Such a cell culture platform can offer novel strategies to obtain functional pancreatic β-cells from patient-specific cell sources, ultimately enabling better treatment for diabetes mellitus. Graphical Abstract: [Figure not available: see fulltext.] © 2023, The Korean Society for Biomaterials. | - |
dc.format.extent | 18 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | BioMed Central Ltd | - |
dc.title | Self-organized insulin-producing β-cells differentiated from human omentum-derived stem cells and their in vivo therapeutic potential | - |
dc.type | Article | - |
dc.publisher.location | 대한민국 | - |
dc.identifier.doi | 10.1186/s40824-023-00419-1 | - |
dc.identifier.scopusid | 2-s2.0-85169006899 | - |
dc.identifier.wosid | 001057789100001 | - |
dc.identifier.bibliographicCitation | Biomaterials Research, v.27, no.1, pp 1 - 18 | - |
dc.citation.title | Biomaterials Research | - |
dc.citation.volume | 27 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 1 | - |
dc.citation.endPage | 18 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Engineering | - |
dc.relation.journalResearchArea | Materials Science | - |
dc.relation.journalWebOfScienceCategory | Engineering, Biomedical | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Biomaterials | - |
dc.subject.keywordPlus | MOLECULE N-CAM | - |
dc.subject.keywordPlus | STROMAL CELLS | - |
dc.subject.keywordPlus | EXTRACELLULAR-MATRIX | - |
dc.subject.keywordPlus | PANCREATIC-ISLETS | - |
dc.subject.keywordPlus | ADHESION | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | CALCIUM | - |
dc.subject.keywordPlus | CLUSTER | - |
dc.subject.keywordPlus | SCAFFOLD | - |
dc.subject.keywordPlus | MARROW | - |
dc.subject.keywordAuthor | Cell adhesion | - |
dc.subject.keywordAuthor | Cell-to-cell interaction | - |
dc.subject.keywordAuthor | Fibroblast growth factor 2 | - |
dc.subject.keywordAuthor | Insulin-producing cells | - |
dc.subject.keywordAuthor | Pancreatic β-cells | - |
dc.subject.keywordAuthor | Stem cell differentiation | - |
dc.subject.keywordAuthor | Streptozotocin-induced diabetic models | - |
dc.identifier.url | https://www.scopus.com/record/display.uri?eid=2-s2.0-85169006899&origin=inward&txGid=e6eeb3857eca8e66337b348a0c8f62df | - |
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