Defined Conditions Control the Morphological Dualism of Rat Primitive Extraembryonic Endoderm Stem Cells
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Wang, Xiaoqiong | - |
dc.contributor.author | Kim, Minjae | - |
dc.contributor.author | Jung, Kyoung Hwa | - |
dc.contributor.author | Chai, Young Gyu | - |
dc.contributor.author | Binas, Bert | - |
dc.date.accessioned | 2023-12-08T09:33:40Z | - |
dc.date.available | 2023-12-08T09:33:40Z | - |
dc.date.issued | 2023-12 | - |
dc.identifier.issn | 1547-3287 | - |
dc.identifier.issn | 1557-8534 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/115955 | - |
dc.description.abstract | Rat primitive extraembryonic endoderm (pXEN) stem cell lines indefinitely preserve the characteristic features of the early extraembryonic endoderm (ExEn) in vitro, but require unknown serum factors and exhibit a hybrid (mesenchymal-epithelial) phenotype. We report two chemically defined conditions that differ by the addition of the cytokine leukemia inhibitory factor (Lif) and the b-catenin-stabilizing drug Chir99021, and enable permanent self-renewal as mesenchymal and epithelial morphotypes, respectively. The morphotypes are interconvertible and equipotent, as shown by the formation of well-differentiated organoids. Surprisingly, the proliferation of both morphotypes requires Lif-type Gp130/Stat3 signaling (autocrine in the absence of added Lif) and noncanonical Wnt signaling (autocrine). In addition, the epithelial version requires b-catenin for proliferation and morphology. Interestingly, the mesenchymal cells also express key epithelial markers, but those are improperly structured and/or not functional, indicating a primed state. These results provide an improved platform for studying the proliferation and plasticity of the early ExEn, which occurs in mesenchymal and epithelial forms in vivo. © 2023 Mary Ann Liebert Inc.. All rights reserved. | - |
dc.format.extent | 16 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Mary Ann Liebert Inc. | - |
dc.title | Defined Conditions Control the Morphological Dualism of Rat Primitive Extraembryonic Endoderm Stem Cells | - |
dc.type | Article | - |
dc.publisher.location | 미국 | - |
dc.identifier.doi | 10.1089/scd.2023.0187 | - |
dc.identifier.scopusid | 2-s2.0-85177242239 | - |
dc.identifier.wosid | 001112552200002 | - |
dc.identifier.bibliographicCitation | Stem Cells and Development, v.32, no.23-24, pp 1 - 16 | - |
dc.citation.title | Stem Cells and Development | - |
dc.citation.volume | 32 | - |
dc.citation.number | 23-24 | - |
dc.citation.startPage | 1 | - |
dc.citation.endPage | 16 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalResearchArea | Hematology | - |
dc.relation.journalResearchArea | Research & Experimental Medicine | - |
dc.relation.journalResearchArea | Transplantation | - |
dc.relation.journalWebOfScienceCategory | Cell & Tissue Engineering | - |
dc.relation.journalWebOfScienceCategory | Hematology | - |
dc.relation.journalWebOfScienceCategory | Medicine, Research & Experimental | - |
dc.relation.journalWebOfScienceCategory | Transplantation | - |
dc.subject.keywordPlus | XEN CELLS | - |
dc.subject.keywordPlus | VISCERAL ENDODERM | - |
dc.subject.keywordPlus | DIFFERENTIATION | - |
dc.subject.keywordPlus | PLURIPOTENCY | - |
dc.subject.keywordAuthor | chemically defined culture | - |
dc.subject.keywordAuthor | EMT/MET | - |
dc.subject.keywordAuthor | extraembryonic endoderm | - |
dc.subject.keywordAuthor | rat | - |
dc.subject.keywordAuthor | stem cells | - |
dc.identifier.url | https://www.liebertpub.com/doi/10.1089/scd.2023.0187 | - |
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