Streamlined measurement of chimeric antigen receptor T-cell concentration, size, viability and two-color phenotyping during manufacturing
DC Field | Value | Language |
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dc.contributor.author | Pajarillo, Raymone | - |
dc.contributor.author | Paruzzo, Luca | - |
dc.contributor.author | Carturan, Alberto | - |
dc.contributor.author | Ugwuanyi, Ositadimma | - |
dc.contributor.author | White, Griffin | - |
dc.contributor.author | Guruprasad, Puneeth | - |
dc.contributor.author | Ballard, Hatcher J | - |
dc.contributor.author | Patel, Ruchi P | - |
dc.contributor.author | Zhang, Yunlin | - |
dc.contributor.author | Lee, Yong Gu | - |
dc.contributor.author | Hong, Seok Jae Albert | - |
dc.contributor.author | Dittami, Gregory M. | - |
dc.contributor.author | Ruella, Marco | - |
dc.date.accessioned | 2024-04-01T08:30:35Z | - |
dc.date.available | 2024-04-01T08:30:35Z | - |
dc.date.issued | 2024-05 | - |
dc.identifier.issn | 1465-3249 | - |
dc.identifier.issn | 1477-2566 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/118344 | - |
dc.description.abstract | Background aims: The successful development of CD19-targeted chimeric antigen receptor (CAR) T-cell therapies has led to an exponential increase in the number of patients recieving treatment and the advancement of novel CAR T products. Therefore, there is a strong need to develop streamlined platforms that allow rapid, cost-effective, and accurate measurement of the key characteristics of CAR T cells during manufacturing (i.e., cell number, cell size, viability, and basic phenotype). Methods: In this study, we compared the novel benchtop cell analyzer Moxi GO II (ORFLO Technologies), which enables simultaneous evaluation of all the aforementioned parameters, with current gold standards in the field: the Multisizer Coulter Counter (cell counter) and the BD LSRFortessa (flow cytometer). Results: Our results demonstrated that the Moxi GO II can accurately measure cell number and cell size (i.e., cell volume) while simultaneously assessing simple two-color flow cytometry parameters, such as CAR T-cell viability and CD4 or CAR expression. Conclusions: These measurements are comparable with those of gold standard instruments, demonstrating that the Moxi GO II is a promising platform for quickly monitoring CAR T-cell growth and phenotype in research-grade and clinical samples. © 2024 International Society for Cell & Gene Therapy | - |
dc.format.extent | 6 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Taylor & Francis | - |
dc.title | Streamlined measurement of chimeric antigen receptor T-cell concentration, size, viability and two-color phenotyping during manufacturing | - |
dc.type | Article | - |
dc.publisher.location | 영국 | - |
dc.identifier.doi | 10.1016/j.jcyt.2024.01.007 | - |
dc.identifier.scopusid | 2-s2.0-85187644999 | - |
dc.identifier.wosid | 001237218300001 | - |
dc.identifier.bibliographicCitation | Cytotherapy, v.26, no.5, pp 1 - 6 | - |
dc.citation.title | Cytotherapy | - |
dc.citation.volume | 26 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 1 | - |
dc.citation.endPage | 6 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Hematology | - |
dc.relation.journalResearchArea | Research & Experimental Medicine | - |
dc.relation.journalWebOfScienceCategory | Cell & Tissue Engineering | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Hematology | - |
dc.relation.journalWebOfScienceCategory | Medicine, Research & Experimental | - |
dc.subject.keywordAuthor | CAR T-cell manufacturing | - |
dc.subject.keywordAuthor | cellular Immunotherapies | - |
dc.subject.keywordAuthor | flow cytometry | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S1465324924000240?via%3Dihub | - |
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