Ultrasensitive and Rapid Circulating Tumor DNA Liquid Biopsy Using Surface-Confined Gene Amplification on Dispersible Magnetic Nano-Electrodes
- Authors
- Park, Bum Chul; Soh, Jeong Ook; Choi, Hee-Joo; Park, Hyeon Su; Lee, Sang Min; Fu, Hong En; Kim, Myeong Soo; Ko, Min Jun; Koo, Thomas Myeongseok; Lee, Jeong-Yeon; Kim, Young Keun; Lee, Ju Hun
- Issue Date
- May-2024
- Publisher
- American Chemical Society
- Keywords
- circulating tumor DNA; liquid biopsy; geneamplification; electrochemical detection; magneticnanoparticles; surface functionalization; superparamagnetism
- Citation
- ACS Nano, v.18, no.20, pp 12781 - 12794
- Pages
- 14
- Indexed
- SCIE
SCOPUS
- Journal Title
- ACS Nano
- Volume
- 18
- Number
- 20
- Start Page
- 12781
- End Page
- 12794
- URI
- https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/119239
- DOI
- 10.1021/acsnano.3c12266
- ISSN
- 1936-0851
1936-086X
- Abstract
- Circulating tumor DNA (ctDNA) detection has been acknowledged as a promising liquid biopsy approach for cancer diagnosis, with various ctDNA assays used for early detection and treatment monitoring. Dispersible magnetic nanoparticle-based electrochemical detection methods have been proposed as promising candidates for ctDNA detection based on the detection performance and features of the platform material. This study proposes a nanoparticle surface-localized genetic amplification approach by integrating Fe3O4-Au core-shell nanoparticles into polymerase chain reactions (PCR). These highly dispersible and magnetically responsive superparamagnetic nanoparticles act as nano-electrodes that amplify and accumulate target ctDNA in situ on the nanoparticle surface upon PCR amplification. These nanoparticles are subsequently captured and subjected to repetitive electrochemical measurements to induce reconfiguration-mediated signal amplification for ultrasensitive (similar to 3 aM) and rapid (similar to 7 min) metastatic breast cancer ctDNA detection in vitro. The detection platform can also detect metastatic biomarkers from in vivo samples, highlighting the potential for clinical applications and further expansion to rapid and ultrasensitive multiplex detection of various cancers.
- Files in This Item
-
Go to Link
- Appears in
Collections - COLLEGE OF ENGINEERING SCIENCES > DEPARTMENT OF BIONANO ENGINEERING > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.