골격근 감소 개선 효과를 가지는 생지황의 성분 및 생지황 건조과정에서 주요 성분의 상대 함량 비교

Abstract

The fresh roots extract of Rehmannia glutinosa showed a preventive effect in a dexamethasone-induced C2C12 myotube atrophy model. Bioactivity-guided isolation using centrifugal partition chromatography and prep-HPLC yielded to ten compounds: five phenylethanoid glycosides (1, 2, 4, 5, and 7), three iridoids (6, 9, and 10), one phenylpropanoid (3) and one sesquiterpene glycoside (8). Their chemical structures were elucidated as echinacoside (1), jionoside A1 (2), ferulic acid (3), verbascoside (4), jinoside B1 (5), 6-O-E-feruloyl ajugol (6), martynoside (7), aeginetic acid 5-O-β-D-quinovoside (8), aegine-toyl ajugol 5-O-β-D-qunovoside (9), and rehmaglutinoside F (10) by spectroscopic analysis. Among them, compounds 7 and 9 demonstrated preventive effects against dexamethasone-induced skeletal muscle atrophy in C2C12 myotubes. Furthermore, the during the drying process of fresh R. glutinosa, the relative content ratio of compound 6 increased by 7.8 fold, while the levels of compounds 5, 6, 8, and 9 decreased by more than 50%. © 2024, Korean Society of Pharmacognosy. All rights reserved.