Human autologous iPSC-derived dopaminergic progenitors restore motor function in Parkinson's disease models
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Song, Bin | - |
dc.contributor.author | Cha, Young | - |
dc.contributor.author | Ko, Sanghyeok | - |
dc.contributor.author | Jeon, Jeha | - |
dc.contributor.author | Lee, Nayeon | - |
dc.contributor.author | Seo, Hyemyung | - |
dc.contributor.author | Park, Kyung-Joon | - |
dc.contributor.author | Lee, In-Hee | - |
dc.contributor.author | Lopes, Claudia | - |
dc.contributor.author | Feitosa, Melissa | - |
dc.contributor.author | Luna, Maria Jose | - |
dc.contributor.author | Jung, Jin Hyuk | - |
dc.contributor.author | Kim, Jisun | - |
dc.contributor.author | Hwang, Dabin | - |
dc.contributor.author | Cohen, Bruce M. | - |
dc.contributor.author | Teicher, Martin H. | - |
dc.contributor.author | Leblanc, Pierre | - |
dc.contributor.author | Carter, Bob S. | - |
dc.contributor.author | Kordower, Jeffrey H. | - |
dc.contributor.author | Bolshakov, Vadim Y. | - |
dc.contributor.author | Kong, Sek Won | - |
dc.contributor.author | Schweitzer, Jeffrey S. | - |
dc.contributor.author | Kim, Kwang-Soo | - |
dc.date.accessioned | 2021-06-22T09:07:48Z | - |
dc.date.available | 2021-06-22T09:07:48Z | - |
dc.date.created | 2021-01-21 | - |
dc.date.issued | 2020-02 | - |
dc.identifier.issn | 0021-9738 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/1289 | - |
dc.description.abstract | Parkinson's disease (PD) is a neurodegenerative disorder associated with loss of striatal dopamine, secondary to degeneration of midbrain dopamine (mDA) neurons in the substantia nigra, rendering cell transplantation a promising therapeutic strategy. To establish human induced pluripotent stem cell-based (hiPSC-based) autologous cell therapy, we report a platform of core techniques for the production of mDA progenitors as a safe and effective therapeutic product. First, by combining metabolism-regulating microRNAs with reprogramming factors, we developed a method to more efficiently generate clinical-grade iPSCs, as evidenced by genomic integrity and unbiased pluripotent potential. Second, we established a "spotting"-based in vitro differentiation methodology to generate functional and healthy mDA cells in a scalable manner. Third, we developed a chemical method that safely eliminates undifferentiated cells from the final product. Dopaminergic cells thus express high levels of characteristic mDA markers, produce and secrete dopamine, and exhibit electrophysiological features typical of mDA cells. Transplantation of these cells into rodent models of PD robustly restores motor function and reinnervates host brain, while showing no evidence of tumor formation or redistribution of the implanted cells. We propose that this platform is suitable for the successful implementation of human personalized autologous cell therapy for PD. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | American Society for Clinical Investigation | - |
dc.title | Human autologous iPSC-derived dopaminergic progenitors restore motor function in Parkinson's disease models | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Seo, Hyemyung | - |
dc.identifier.doi | 10.1172/JCI130767 | - |
dc.identifier.scopusid | 2-s2.0-85078866695 | - |
dc.identifier.wosid | 000512418100038 | - |
dc.identifier.bibliographicCitation | Journal of Clinical Investigation, v.130, no.2, pp.904 - 920 | - |
dc.relation.isPartOf | Journal of Clinical Investigation | - |
dc.citation.title | Journal of Clinical Investigation | - |
dc.citation.volume | 130 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 904 | - |
dc.citation.endPage | 920 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Research & Experimental Medicine | - |
dc.relation.journalWebOfScienceCategory | Medicine, Research & Experimental | - |
dc.subject.keywordPlus | PLURIPOTENT STEM-CELLS | - |
dc.subject.keywordPlus | CLINICAL TRANSLATION | - |
dc.subject.keywordPlus | RAT MODEL | - |
dc.subject.keywordPlus | HUMAN ES | - |
dc.subject.keywordPlus | ANIMAL-MODELS | - |
dc.subject.keywordPlus | NEURONS | - |
dc.subject.keywordPlus | TRANSPLANTATION | - |
dc.subject.keywordPlus | CANCER | - |
dc.subject.keywordPlus | DIFFERENTIATION | - |
dc.subject.keywordPlus | THERAPY | - |
dc.identifier.url | https://www.jci.org/articles/view/130767 | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
55 Hanyangdeahak-ro, Sangnok-gu, Ansan, Gyeonggi-do, 15588, Korea+82-31-400-4269 sweetbrain@hanyang.ac.kr
COPYRIGHT © 2021 HANYANG UNIVERSITY. ALL RIGHTS RESERVED.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.