Functional Characterization of Exonic Variants of the PPARGC1B Gene in Coregulation of Estrogen Receptor Alpha
DC Field | Value | Language |
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dc.contributor.author | Chang, Hun Soo | - |
dc.contributor.author | Lee, Shin-Hwa | - |
dc.contributor.author | Lee, Jong-Uk | - |
dc.contributor.author | Park, Jong Sook | - |
dc.contributor.author | Chung, Il Yup | - |
dc.contributor.author | Park, Choon-Sik | - |
dc.date.accessioned | 2021-06-22T16:25:23Z | - |
dc.date.available | 2021-06-22T16:25:23Z | - |
dc.date.created | 2021-01-21 | - |
dc.date.issued | 2016-07 | - |
dc.identifier.issn | 1044-5498 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/13211 | - |
dc.description.abstract | Peroxisome proliferator-activated receptor gamma coactivator 1 beta (PPARGC1B) is a coactivator of estrogen receptor (ER)alpha and ER beta. We previously demonstrated a significant association between a variant of exon 5 of the PPARGC1B gene (+102525 G>A, R265Q) and airway hyperreactivity (AHR). The aims of the study were to evaluate the genetic effects of variants of the PPARGC1B gene on the function of ERs. PPARGC1B +102525G and A gene constructs were generated using PCR and cloned into a pCMV4 promoter vector. A luciferase reporter assay was undertaken in 293T cells cotransfected with one of the PPARGC1B +102525G>A constructs, ER alpha, and an estrogen response element (ERE) containing a luciferase construct after treatment with 17 beta-estradiol. According to the luciferase reporter assay, the +102525A allele showed higher ER alpha activity than the +102525G allele in response to stimulation with 17 beta-estradiol. In addition, the interaction between ER alpha and PPARGC1B was evaluated by coprecipitation assay. Human influenza hemagglutinin-tagged PPARGC1B co-precipitated more intensely with ER alpha in the +102525A than the +102525G construct after 17 beta estradiol treatment. The variant +102525A allele enhances the activity of ER alpha to a greater degree than the +102525G allele of PPARGC1B. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | Mary Ann Liebert Inc. | - |
dc.title | Functional Characterization of Exonic Variants of the PPARGC1B Gene in Coregulation of Estrogen Receptor Alpha | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Chung, Il Yup | - |
dc.identifier.doi | 10.1089/dna.2015.3195 | - |
dc.identifier.scopusid | 2-s2.0-84978101085 | - |
dc.identifier.wosid | 000379447800002 | - |
dc.identifier.bibliographicCitation | DNA and Cell Biology, v.35, no.7, pp.314 - 321 | - |
dc.relation.isPartOf | DNA and Cell Biology | - |
dc.citation.title | DNA and Cell Biology | - |
dc.citation.volume | 35 | - |
dc.citation.number | 7 | - |
dc.citation.startPage | 314 | - |
dc.citation.endPage | 321 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalResearchArea | Genetics & Heredity | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Genetics & Heredity | - |
dc.subject.keywordPlus | SINGLE-NUCLEOTIDE POLYMORPHISMS | - |
dc.subject.keywordPlus | COACTIVATOR 1-BETA PGC-1-BETA | - |
dc.subject.keywordPlus | AIRWAY HYPERRESPONSIVENESS | - |
dc.subject.keywordPlus | SMOOTH-MUSCLE | - |
dc.subject.keywordPlus | TRANSCRIPTION FACTORS | - |
dc.subject.keywordPlus | DOWN-REGULATION | - |
dc.subject.keywordPlus | ASTHMA | - |
dc.subject.keywordPlus | ASSOCIATION | - |
dc.subject.keywordPlus | INFLAMMATION | - |
dc.subject.keywordPlus | SUSCEPTIBILITY | - |
dc.identifier.url | https://www.liebertpub.com/doi/10.1089/dna.2015.3195 | - |
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