Bioorthogonal Copper Free Click Chemistry for Labeling and Tracking of Chondrocytes In Vivo
DC Field | Value | Language |
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dc.contributor.author | Yoon, Hwa In | - |
dc.contributor.author | Yhee, Ji Young | - |
dc.contributor.author | Na, Jin Hee | - |
dc.contributor.author | Lee, Sangmin | - |
dc.contributor.author | Lee, Hyukjin | - |
dc.contributor.author | Kang, Sun-Woong | - |
dc.contributor.author | Chang, Hyeyoun | - |
dc.contributor.author | Ryu, Ju Hee | - |
dc.contributor.author | Lee, Seulki | - |
dc.contributor.author | Kwon, Ick Chan | - |
dc.contributor.author | Cho, Yong Woo | - |
dc.contributor.author | Kim, Kwangmeyung | - |
dc.date.accessioned | 2021-06-22T17:03:36Z | - |
dc.date.available | 2021-06-22T17:03:36Z | - |
dc.date.created | 2021-01-21 | - |
dc.date.issued | 2016-04 | - |
dc.identifier.issn | 1043-1802 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/14124 | - |
dc.description.abstract | Establishment of an appropriate cell labeling and tracking method is essential for the development of cell-based therapeutic strategies. Here, we are introducing a new method for cell labeling and tracking by combining metabolic gylcoengineering and bioorthogonal copper-free Click chemistry. First, chondrocytes were treated with tetraacetylated N-azidoacetyl-D-mannosamine (Ac(4)ManNAz) to generate unnatural azide groups (-N-3) on the surface of the cells. Subsequently, the unnatural azide groups on the cell surface were specifically conjugated with near-infrared fluorescent (NIRF) dye-tagged dibenzyl cyclooctyne (DBCO-650) through bioorthogonal copper-free Click chemistry. Importantly, DBCO-650-labeled chondrocytes presented strong NIRF signals with relatively low cytotoxicity and the amounts of azide groups and DBCO-650 could be easily controlled by feeding different amounts of Ac4ManNAz and DBCO-650 to the cell culture system. For the in vivo cell tracking, DBCO-650-labeled chondrocytes (1 x 10(6) cells) seeded on the 3D scaffold were subcutaneously implanted into mice and the transplanted DBCO-650-labeled chondrocytes could be effectively tracked in the prolonged time period of 4 weeks using NIRF imaging technology. Furthermore, this new cell labeling and tracking technology had minimal effect on cartilage formation in vivo. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.title | Bioorthogonal Copper Free Click Chemistry for Labeling and Tracking of Chondrocytes In Vivo | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Cho, Yong Woo | - |
dc.identifier.doi | 10.1021/acs.bioconjchem.6b00010 | - |
dc.identifier.scopusid | 2-s2.0-84965082415 | - |
dc.identifier.wosid | 000374812600011 | - |
dc.identifier.bibliographicCitation | BIOCONJUGATE CHEMISTRY, v.27, no.4, pp.927 - 936 | - |
dc.relation.isPartOf | BIOCONJUGATE CHEMISTRY | - |
dc.citation.title | BIOCONJUGATE CHEMISTRY | - |
dc.citation.volume | 27 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 927 | - |
dc.citation.endPage | 936 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Organic | - |
dc.subject.keywordPlus | STEM-CELLS | - |
dc.subject.keywordPlus | STRATEGIES | - |
dc.subject.keywordPlus | THERAPY | - |
dc.subject.keywordPlus | PROLIFERATION | - |
dc.subject.keywordPlus | NANOPARTICLES | - |
dc.subject.keywordPlus | SELECTIVITY | - |
dc.subject.keywordPlus | CARTILAGE | - |
dc.subject.keywordPlus | SURVIVAL | - |
dc.subject.keywordPlus | DISEASE | - |
dc.subject.keywordAuthor | STEM-CELLS | - |
dc.subject.keywordAuthor | STRATEGIES | - |
dc.subject.keywordAuthor | THERAPY | - |
dc.subject.keywordAuthor | PROLIFERATION | - |
dc.subject.keywordAuthor | NANOPARTICLES | - |
dc.subject.keywordAuthor | SELECTIVITY | - |
dc.subject.keywordAuthor | CARTILAGE | - |
dc.subject.keywordAuthor | SURVIVAL | - |
dc.subject.keywordAuthor | DISEASE | - |
dc.identifier.url | https://pubs.acs.org/doi/10.1021/acs.bioconjchem.6b00010 | - |
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