Highly Branched Pentasaccharide-Bearing Amphiphiles for Membrane Protein Studies
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Ehsan, Muhammad | - |
dc.contributor.author | Du, Yang | - |
dc.contributor.author | Scull, Nicola J. | - |
dc.contributor.author | Tikhonova, Elena | - |
dc.contributor.author | Tarrasch, Jeffrey | - |
dc.contributor.author | Mortensen, Jonas S. | - |
dc.contributor.author | Loland, Claus J. | - |
dc.contributor.author | Skiniotis, Georgios | - |
dc.contributor.author | Guan, Lan | - |
dc.contributor.author | Byrne, Bernadette | - |
dc.contributor.author | Kobilka, Brian K. | - |
dc.contributor.author | Chae, Pil Seok | - |
dc.date.accessioned | 2021-06-22T17:04:08Z | - |
dc.date.available | 2021-06-22T17:04:08Z | - |
dc.date.created | 2021-01-21 | - |
dc.date.issued | 2016-03 | - |
dc.identifier.issn | 0002-7863 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/14145 | - |
dc.description.abstract | Detergents are essential tools for membrane protein manipulation. Micelles formed by detergent molecules have the ability to encapsulate the "hydrophobic domains of membrane proteins. The resulting protein detergent complexes (PDCs) are compatible with the polar environments of aqueous media, making structural and functional analysis feasible. Although a number of novel agents have been developed to overcome the limitations of conventional detergents, most have traditional head groups such as glucoside or maltoside. In this study, we introduce a class of amphiphiles, the PSA/Es with a novel highly branched pentasaccharide hydrophilic group. The PSA/Es conferred markedly increased stability to a diverse range of membrane proteins compared to conventional detergents, indicating a positive role for the new hydrophilic group in maintaining the native protein integrity. In addition, PDCs formed by PSA/Es were smaller and more suitable for electron microscopic analysis than those formed by DDM, indicating that the new agents have significant potential for the structure function studies of membrane proteins. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | American Chemical Society | - |
dc.title | Highly Branched Pentasaccharide-Bearing Amphiphiles for Membrane Protein Studies | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Chae, Pil Seok | - |
dc.identifier.doi | 10.1021/jacs.5b13233 | - |
dc.identifier.scopusid | 2-s2.0-84962074275 | - |
dc.identifier.wosid | 000372854200027 | - |
dc.identifier.bibliographicCitation | Journal of the American Chemical Society, v.138, no.11, pp.3789 - 3796 | - |
dc.relation.isPartOf | Journal of the American Chemical Society | - |
dc.citation.title | Journal of the American Chemical Society | - |
dc.citation.volume | 138 | - |
dc.citation.number | 11 | - |
dc.citation.startPage | 3789 | - |
dc.citation.endPage | 3796 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.subject.keywordPlus | CRYSTAL-STRUCTURE | - |
dc.subject.keywordPlus | FACIAL AMPHIPHILES | - |
dc.subject.keywordPlus | BETA(2)-ADRENERGIC RECEPTOR | - |
dc.subject.keywordPlus | STRUCTURAL INSIGHTS | - |
dc.subject.keywordPlus | STABILIZATION | - |
dc.subject.keywordPlus | CRYSTALLIZATION | - |
dc.subject.keywordPlus | DETERGENTS | - |
dc.subject.keywordPlus | SOLUBILIZATION | - |
dc.subject.keywordPlus | COMPLEX | - |
dc.subject.keywordPlus | FLUORESCENCE | - |
dc.identifier.url | https://pubs.acs.org/doi/10.1021/jacs.5b13233 | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
55 Hanyangdeahak-ro, Sangnok-gu, Ansan, Gyeonggi-do, 15588, Korea+82-31-400-4269 sweetbrain@hanyang.ac.kr
COPYRIGHT © 2021 HANYANG UNIVERSITY. ALL RIGHTS RESERVED.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.