Induction of methionine adenosyltransferase 2A in tamoxifen-resistant breast cancer cells
DC Field | Value | Language |
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dc.contributor.author | Nguyen Thi Thuy Phuong | - |
dc.contributor.author | Kim, Sang Kyum | - |
dc.contributor.author | Im, Ji Hye | - |
dc.contributor.author | Yang, Jin Won | - |
dc.contributor.author | Choi, Min Chang | - |
dc.contributor.author | Lim, Sung Chul | - |
dc.contributor.author | Lee, Kwang Yeol | - |
dc.contributor.author | Kim, Young-Mi | - |
dc.contributor.author | Yoon, Jeong Hoon | - |
dc.contributor.author | Kang, Keon Wook | - |
dc.date.accessioned | 2021-06-22T17:04:10Z | - |
dc.date.available | 2021-06-22T17:04:10Z | - |
dc.date.created | 2021-01-21 | - |
dc.date.issued | 2016-03 | - |
dc.identifier.issn | 1949-2553 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/14146 | - |
dc.description.abstract | We previously showed that S-adenosylmethionine-mediated hypermethylation of the PTEN promoter was important for the growth of tamoxifen-resistant MCF-7 (TAMR-MCF-7) cancer cells. Here, we found that the basal expression level of methionine adenosyltransferase 2A (MAT2A), a critical enzyme for the biosynthesis of S-adenosylmethionine, was up-regulated in TAMR-MCF-7 cells compared with control MCF-7 cells. Moreover, the basal expression level of MAT2A in T47D cells, a TAM-resistant estrogen receptor-positive cell line was higher compared to MCF-7 cells. Immunohistochemistry confirmed that MAT2A expression in TAM-resistant human breast cancer tissues was higher than that in TAM-responsive cases. The promoter region of human MAT2A contains binding sites for nuclear factor-kappa B, activator protein-1 (AP-1), and NF-E2-related factor 2 (Nrf2), and the activities of these three transcription factors were enhanced in TAMR-MCF-7 cells. Both the protein expression and transcriptional activity of MAT2A in TAMR-MCF-7 cells were potently suppressed by NF-kappa B inhibition but not by c-Jun/AP-1 or Nrf2 knock-down. Interestingly, the expression levels of microRNA (miR)-146a and -146b were diminished in TAMR-MCF-7 cells, and miR-146b transduction decreased NF-kappa B-mediated MAT2A expression. miR-146b restored PTEN expression via the suppression of PTEN promoter methylation in TAMR-MCF-7 cells. Additionally, miR-146b overexpression inhibited cell proliferation and reversed chemoresistance to 4-hydroxytamoxifen in TAMR-MCF-7 cells. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | IMPACT JOURNALS LLC | - |
dc.title | Induction of methionine adenosyltransferase 2A in tamoxifen-resistant breast cancer cells | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Young-Mi | - |
dc.identifier.doi | 10.18632/oncotarget.5298 | - |
dc.identifier.scopusid | 2-s2.0-84971654738 | - |
dc.identifier.wosid | 000375687200048 | - |
dc.identifier.bibliographicCitation | ONCOTARGET, v.7, no.12, pp.13902 - 13916 | - |
dc.relation.isPartOf | ONCOTARGET | - |
dc.citation.title | ONCOTARGET | - |
dc.citation.volume | 7 | - |
dc.citation.number | 12 | - |
dc.citation.startPage | 13902 | - |
dc.citation.endPage | 13916 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Oncology | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Oncology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.subject.keywordPlus | NF-KAPPA-B | - |
dc.subject.keywordPlus | ADENOSYL-L-METHIONINE | - |
dc.subject.keywordPlus | HUMAN LIVER-CANCER | - |
dc.subject.keywordPlus | S-ADENOSYLMETHIONINE | - |
dc.subject.keywordPlus | PROMOTER HYPERMETHYLATION | - |
dc.subject.keywordPlus | GASTRIC-CARCINOMA | - |
dc.subject.keywordPlus | MCF-7 CELLS | - |
dc.subject.keywordPlus | GROWTH | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordAuthor | tamoxifen resistance | - |
dc.subject.keywordAuthor | miR-146b | - |
dc.subject.keywordAuthor | MAT2A | - |
dc.subject.keywordAuthor | NF-kappa B | - |
dc.subject.keywordAuthor | PTEN | - |
dc.identifier.url | https://www.oncotarget.com/article/5298/text/ | - |
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