Development of a hydrophilic interaction liquid chromatography-tandem mass spectrometric method for the determination of kinsenoside, an antihyperlipidemic candidate, in rat plasma and its application to pharmacokinetic studies
DC Field | Value | Language |
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dc.contributor.author | Rehman, Shaheed Ur | - |
dc.contributor.author | Kim, In Sook | - |
dc.contributor.author | Choi, Min Sun | - |
dc.contributor.author | Luo, Zengwei | - |
dc.contributor.author | Yao, Guangming | - |
dc.contributor.author | Xue, Yongbo | - |
dc.contributor.author | Zhang, Yonghui | - |
dc.contributor.author | Yoo, Hye Hyun | - |
dc.date.accessioned | 2021-06-22T17:21:34Z | - |
dc.date.available | 2021-06-22T17:21:34Z | - |
dc.date.created | 2021-01-21 | - |
dc.date.issued | 2016-02 | - |
dc.identifier.issn | 0731-7085 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/14515 | - |
dc.description.abstract | Kinsenoside is a major bioactive constituent isolated from Anoectochilus formosanus and is investigated as an antihyperlipidemic candidate. In this study, a rapid, sensitive, and reliable bioanalytical method was developed for the determination of kinsenoside in rat plasma using hydrophilic interaction liquid chromatography-tandem mass spectrometry (HILIC-MS/MS). The plasma sample was pretreated with 1% acetic acid, followed by protein precipitation with acetonitrile:methanol (70:30). Chromatographic separation was performed on a HILIC silica column (2.1 mm x 100 mm, 3 mu m). The mobile phases consisted of 0.1% acetic acid in distilled water (solvent A) and 0.1% acetic acid in acetonitrile (solvent B). A gradient program was used at a flow rate of 0.2 mi./min. For mass spectrometric detection, the multiple reaction monitoring mode was used; the MRM transitions were m/z 265.2 -> m/z 102.9 for kinsenoside and m/z 1633 -> m/z 132.1 for the internal standard (IS) nicotine in the positive ionization mode. A calibration curve was constructed in the range of 2-500 ng/mL. The intra- and interday precision and accuracy were within 5%. The HILIC-MS/MS method was specific, accurate, and reproducible and was successfully applied in a pharmacokinetic study of kinsenoside in rats. (C) 2015 Elsevier B.V. All rights reserved. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | Elsevier BV | - |
dc.title | Development of a hydrophilic interaction liquid chromatography-tandem mass spectrometric method for the determination of kinsenoside, an antihyperlipidemic candidate, in rat plasma and its application to pharmacokinetic studies | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Yoo, Hye Hyun | - |
dc.identifier.doi | 10.1016/j.jpba.2015.12.003 | - |
dc.identifier.scopusid | 2-s2.0-84949604812 | - |
dc.identifier.wosid | 000370585700003 | - |
dc.identifier.bibliographicCitation | Journal of Pharmaceutical and Biomedical Analysis, v.120, pp.19 - 24 | - |
dc.relation.isPartOf | Journal of Pharmaceutical and Biomedical Analysis | - |
dc.citation.title | Journal of Pharmaceutical and Biomedical Analysis | - |
dc.citation.volume | 120 | - |
dc.citation.startPage | 19 | - |
dc.citation.endPage | 24 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.subject.keywordPlus | ANOECTOCHILUS-FORMOSANUS | - |
dc.subject.keywordPlus | SIMVASTATIN | - |
dc.subject.keywordPlus | STRATEGIES | - |
dc.subject.keywordPlus | ROXBURGHII | - |
dc.subject.keywordAuthor | Kinsenoside | - |
dc.subject.keywordAuthor | Antihyperlipidemic agent | - |
dc.subject.keywordAuthor | LC-MS/MS | - |
dc.subject.keywordAuthor | Plasma | - |
dc.subject.keywordAuthor | Pharmacokinetics | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S0731708515302685?via%3Dihub | - |
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