p21(CIP1) Induces Apoptosis via Binding to BCL2 in LNCaP Prostate Cancer Cells Treated with MCS-C3, A Novel Carbocyclic Analog of Pyrrolopyrimidine
DC Field | Value | Language |
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dc.contributor.author | Choi, Ko-Woon | - |
dc.contributor.author | Suh, Hyewon | - |
dc.contributor.author | Oh, Ha Lim | - |
dc.contributor.author | Ryou, Chongsuk | - |
dc.contributor.author | Lee, Chul-Hoon | - |
dc.date.accessioned | 2021-06-22T17:24:24Z | - |
dc.date.available | 2021-06-22T17:24:24Z | - |
dc.date.created | 2021-01-21 | - |
dc.date.issued | 2016-01 | - |
dc.identifier.issn | 0250-7005 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/14641 | - |
dc.description.abstract | Background: Previously, we synthesized a new carbocyclic analog of pyrrolo[2,3-d]pyrimidine nucleoside, designated MCS-C3. Recently, we found that LNCaP androgen-responsive prostate cancer cells treated with MCS-C3 rapidly undergo intrinsic apoptosis through dramatic up-regulation of p21(CIP1). The present study aimed to evaluate the cellular functions and underlying molecular mechanisms of p21(CIP1) on apoptotic induction in LNCaP cells treated with 6 mu M MCS-C3. Materials and Methods: Western blots, flow cytometric assay, immunoprecipitation, and transmission electron microscopy analysis were used to measure apoptotic induction in 6-mu M MCS-C3-treated LNCaP cells. Effects of MCS-C3 on gene expression of p21(CIP1) were measured by semi-quantitative real-time polymerase chain reaction, and small interfering RNA transfection. Results: MCS-C3 induced appreciable caspase-dependent apoptosis associated with the significant up-regulation of p53-dependent p21(CIP1) in LNCaP cells. Moreover, this apoptotic induction was caused by direct binding of p21(CIP1) to anti-apoptotic B-cell lymphoma 2 (BCL2) protein, and antagonizing BCL2 function. In addition, MCS-C3-mediated apoptotic induction, and up-regulation of p21(CIP1) were almost completely blocked by the treatment of androgen-esponsive LNCaP cells with flutamide, an androgen receptor (AR) antagonist. Conclusion: We identified that induction of intrinsic apoptosis in LNCaP cells by 6 mu M MCS-C3 is associated not only with p53 activation but also with mediation of AR. In the present study, we identified the cellular functions and underlying molecular mechanisms of p53-dependent and AR-associated p21(CIP1) on apoptotic induction via direct binding to BCL2 in LNCaP cells treated with 6 mu M MCS-C3. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | International Institute of Anticancer Research | - |
dc.title | p21(CIP1) Induces Apoptosis via Binding to BCL2 in LNCaP Prostate Cancer Cells Treated with MCS-C3, A Novel Carbocyclic Analog of Pyrrolopyrimidine | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Ryou, Chongsuk | - |
dc.contributor.affiliatedAuthor | Lee, Chul-Hoon | - |
dc.identifier.wosid | 000367450000026 | - |
dc.identifier.bibliographicCitation | Anticancer Research, v.36, no.1, pp.213 - 220 | - |
dc.relation.isPartOf | Anticancer Research | - |
dc.citation.title | Anticancer Research | - |
dc.citation.volume | 36 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 213 | - |
dc.citation.endPage | 220 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Oncology | - |
dc.relation.journalWebOfScienceCategory | Oncology | - |
dc.subject.keywordPlus | ANDROGEN RECEPTOR | - |
dc.subject.keywordPlus | P21 | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | P53 | - |
dc.subject.keywordPlus | INDUCTION | - |
dc.subject.keywordPlus | MITOCHONDRIA | - |
dc.subject.keywordPlus | SANGIVAMYCIN | - |
dc.subject.keywordPlus | ASSOCIATION | - |
dc.subject.keywordPlus | INHIBITOR | - |
dc.subject.keywordPlus | CLEAVAGE | - |
dc.subject.keywordAuthor | Prostate cancer | - |
dc.subject.keywordAuthor | LNCaP cells | - |
dc.subject.keywordAuthor | apoptosis | - |
dc.subject.keywordAuthor | p21(CIP1) | - |
dc.subject.keywordAuthor | BCL2 | - |
dc.identifier.url | https://ar.iiarjournals.org/content/36/1/213/tab-article-info | - |
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