p21CIP1 Induces Apoptosis via Binding to BCL2 in LNCaP Prostate Cancer Cells Treated with MCS-C3, A Novel Carbocyclic Analog of Pyrrolopyrimidine
DC Field | Value | Language |
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dc.contributor.author | Choi, K.-W. | - |
dc.contributor.author | Suh, H. | - |
dc.contributor.author | Oh, H.L. | - |
dc.contributor.author | Ryou, C. | - |
dc.contributor.author | Lee, C.-H. | - |
dc.date.accessioned | 2021-06-22T17:25:04Z | - |
dc.date.available | 2021-06-22T17:25:04Z | - |
dc.date.created | 2021-05-11 | - |
dc.date.issued | 2016-07 | - |
dc.identifier.issn | 0250-7005 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/14670 | - |
dc.description.abstract | BACKGROUND: Previously, we synthesized a new carbocyclic analog of pyrrolo[2,3-d]pyrimidine nucleoside, designated MCS-C3. Recently, we found that LNCaP androgen-responsive prostate cancer cells treated with MCS-C3 rapidly undergo intrinsic apoptosis through dramatic up-regulation of p21(CIP1). The present study aimed to evaluate the cellular functions and underlying molecular mechanisms of p21(CIP1) on apoptotic induction in LNCaP cells treated with 6 μM MCS-C3.MATERIALS AND METHODS: Western blots, flow cytometric assay, immunoprecipitation, and transmission electron microscopy analysis were used to measure apoptotic induction in 6-μM MCS-C3-treated LNCaP cells. Effects of MCS-C3 on gene expression of p21(CIP1) were measured by semi-quantitative real-time polymerase chain reaction, and small interfering RNA transfection.RESULTS: MCS-C3 induced appreciable caspase-dependent apoptosis associated with the significant up-regulation of p53-dependent p21(CIP1) in LNCaP cells. Moreover, this apoptotic induction was caused by direct binding of p21(CIP1) to anti-apoptotic B-cell lymphoma 2 (BCL2) protein, and antagonizing BCL2 function. In addition, MCS-C3-mediated apoptotic induction, and up-regulation of p21(CIP1) were almost completely blocked by the treatment of androgen-responsive LNCaP cells with flutamide, an androgen receptor (AR) antagonist.CONCLUSION: We identified that induction of intrinsic apoptosis in LNCaP cells by 6 μM MCS-C3 is associated not only with p53 activation but also with mediation of AR. In the present study, we identified the cellular functions and underlying molecular mechanisms of p53-dependent and AR-associated p21(CIP1) on apoptotic induction via direct binding to BCL2 in LNCaP cells treated with 6 μM MCS-C3. Copyright© 2016 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.title | p21CIP1 Induces Apoptosis via Binding to BCL2 in LNCaP Prostate Cancer Cells Treated with MCS-C3, A Novel Carbocyclic Analog of Pyrrolopyrimidine | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Ryou, C. | - |
dc.contributor.affiliatedAuthor | Lee, C.-H. | - |
dc.identifier.scopusid | 2-s2.0-84973430219 | - |
dc.identifier.bibliographicCitation | Anticancer research, v.36, no.1, pp.213 - 220 | - |
dc.relation.isPartOf | Anticancer research | - |
dc.citation.title | Anticancer research | - |
dc.citation.volume | 36 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 213 | - |
dc.citation.endPage | 220 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordPlus | BCL2 protein, human | - |
dc.subject.keywordPlus | protein bcl 2 | - |
dc.subject.keywordPlus | pyrimidine derivative | - |
dc.subject.keywordPlus | pyrrole derivative | - |
dc.subject.keywordPlus | pyrrolopyrimidine | - |
dc.subject.keywordPlus | apoptosis | - |
dc.subject.keywordPlus | cell proliferation | - |
dc.subject.keywordPlus | genetic transfection | - |
dc.subject.keywordPlus | human | - |
dc.subject.keywordPlus | immunology | - |
dc.subject.keywordPlus | male | - |
dc.subject.keywordPlus | metabolism | - |
dc.subject.keywordPlus | pathology | - |
dc.subject.keywordPlus | Prostatic Neoplasms | - |
dc.subject.keywordPlus | signal transduction | - |
dc.subject.keywordPlus | tumor cell line | - |
dc.subject.keywordPlus | Apoptosis | - |
dc.subject.keywordPlus | Cell Line, Tumor | - |
dc.subject.keywordPlus | Cell Proliferation | - |
dc.subject.keywordPlus | Humans | - |
dc.subject.keywordPlus | Male | - |
dc.subject.keywordPlus | Prostatic Neoplasms | - |
dc.subject.keywordPlus | Proto-Oncogene Proteins c-bcl-2 | - |
dc.subject.keywordPlus | Pyrimidines | - |
dc.subject.keywordPlus | Pyrroles | - |
dc.subject.keywordPlus | Signal Transduction | - |
dc.subject.keywordPlus | Transfection | - |
dc.subject.keywordAuthor | apoptosis | - |
dc.subject.keywordAuthor | BCL2 | - |
dc.subject.keywordAuthor | LNCaP cells | - |
dc.subject.keywordAuthor | p21CIP1 | - |
dc.subject.keywordAuthor | Prostate cancer | - |
dc.identifier.url | https://ar.iiarjournals.org/content/36/1/213.short | - |
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