Proteolytic disassembly of peptide-mediated graphene oxide assemblies for turn-on fluorescence sensing of proteases
DC Field | Value | Language |
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dc.contributor.author | Yang, Jin-Kyoung | - |
dc.contributor.author | Kwak, Seon-Yeong | - |
dc.contributor.author | Jeon, Su-Ji | - |
dc.contributor.author | Lee, Eunjin | - |
dc.contributor.author | Ju, Jong-Min | - |
dc.contributor.author | Kim, Hye-In | - |
dc.contributor.author | Lee, Yoon-Sik | - |
dc.contributor.author | Kim, Jong-Ho | - |
dc.date.accessioned | 2021-06-22T18:23:01Z | - |
dc.date.available | 2021-06-22T18:23:01Z | - |
dc.date.created | 2021-01-21 | - |
dc.date.issued | 2016-05 | - |
dc.identifier.issn | 2040-3364 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/16028 | - |
dc.description.abstract | Molecule-induced assembly of nanomaterials can alter their unique chemical and physical properties, which can be a promising approach for sensing. Herein, we demonstrate an optical 'turn-on' biosensor for the detection of matrix metalloproteinase-2 (MMP-2), fabricated by means of a peptide-induced assembly of fluorescent graphene oxide (GO). Functionalization of GO with a peptide substrate for MMP-2 bearing a thiol group leads to its self-assembly via disulfide bonding, accompanied by self-quenching of GO's strong fluorescence. This peptide-induced GO assembly is then disassembled by proteolytic cleavage in the presence of MMP-2, thereby restoring the level of self-quenched GO fluorescence. With this approach, we are able to detect MMP-2 and to investigate the kinetic parameters of MMP-2 activity. The GO-peptide assembly is successfully applied to the selective and sensitive detection of MMP-2 secreted by living cells, human hepatocytes HepG2, at a concentration of 2 ng mL(-1). | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | Royal Society of Chemistry | - |
dc.title | Proteolytic disassembly of peptide-mediated graphene oxide assemblies for turn-on fluorescence sensing of proteases | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Jong-Ho | - |
dc.identifier.doi | 10.1039/c6nr02815b | - |
dc.identifier.scopusid | 2-s2.0-84975462036 | - |
dc.identifier.wosid | 000378244900021 | - |
dc.identifier.bibliographicCitation | Nanoscale, v.8, no.24, pp.12272 - 12281 | - |
dc.relation.isPartOf | Nanoscale | - |
dc.citation.title | Nanoscale | - |
dc.citation.volume | 8 | - |
dc.citation.number | 24 | - |
dc.citation.startPage | 12272 | - |
dc.citation.endPage | 12281 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalResearchArea | Materials Science | - |
dc.relation.journalResearchArea | Physics | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Physics, Applied | - |
dc.subject.keywordPlus | RESONANCE ENERGY-TRANSFER | - |
dc.subject.keywordPlus | HIGHLY SENSITIVE DETECTION | - |
dc.subject.keywordPlus | MATRIX METALLOPROTEINASES | - |
dc.subject.keywordPlus | IN-VIVO | - |
dc.subject.keywordPlus | OPTICAL-DETECTION | - |
dc.subject.keywordPlus | SENSOR | - |
dc.subject.keywordPlus | NANOPARTICLES | - |
dc.subject.keywordPlus | GELATINASE | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | BIOSENSOR | - |
dc.subject.keywordAuthor | RESONANCE ENERGY-TRANSFER | - |
dc.subject.keywordAuthor | HIGHLY SENSITIVE DETECTION | - |
dc.subject.keywordAuthor | MATRIX METALLOPROTEINASE-2 | - |
dc.subject.keywordAuthor | IN-VIVO | - |
dc.subject.keywordAuthor | OPTICAL-DETECTION | - |
dc.subject.keywordAuthor | CARCINOMA CELLS | - |
dc.subject.keywordAuthor | QUANTUM DOTS | - |
dc.subject.keywordAuthor | BIOSENSOR | - |
dc.subject.keywordAuthor | SENSOR | - |
dc.subject.keywordAuthor | PROBE | - |
dc.identifier.url | https://pubs.rsc.org/en/content/articlelanding/2016/NR/C6NR02815B | - |
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