A class of rigid linker-bearing glucosides for membrane protein structural study
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Sadaf, Aiman | - |
dc.contributor.author | Mortensen, Jonas S. | - |
dc.contributor.author | Capaldi, Stefano | - |
dc.contributor.author | Tikhonova, Elena | - |
dc.contributor.author | Hariharan, Parameswaran | - |
dc.contributor.author | Ribeiro, Orquidea | - |
dc.contributor.author | Loland, Claus J. | - |
dc.contributor.author | Guan, Lan | - |
dc.contributor.author | Byrne, Bernadette | - |
dc.contributor.author | Chae, Pil Seok | - |
dc.date.accessioned | 2021-06-22T18:28:06Z | - |
dc.date.available | 2021-06-22T18:28:06Z | - |
dc.date.created | 2021-01-21 | - |
dc.date.issued | 2016-03 | - |
dc.identifier.issn | 2041-6520 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/16050 | - |
dc.description.abstract | Membrane proteins are amphipathic bio-macromolecules incompatible with the polar environments of aqueous media. Conventional detergents encapsulate the hydrophobic surfaces of membrane proteins allowing them to exist in aqueous solution. Membrane proteins stabilized by detergent micelles are used for structural and functional analysis. Despite the availability of a large number of detergents, only a few agents are sufficiently effective at maintaining the integrity of membrane proteins to allow successful crystallization. In the present study, we describe a novel class of synthetic amphiphiles with a branched tail group and a triglucoside head group. These head and tail groups were connected via an amide or ether linkage by using a tris(hydroxylmethyl) aminomethane (TRIS) or neopentyl glycol (NPG) linker to produce TRIS-derived triglucosides (TDTs) and NPG-derived triglucosides (NDTs), respectively. Members of this class conferred enhanced stability on target membrane proteins compared to conventional detergents. Because of straightforward synthesis of the novel agents and their favourable effects on a range of membrane proteins, these agents should be of wide applicability to membrane protein science. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | Royal Society of Chemistry | - |
dc.title | A class of rigid linker-bearing glucosides for membrane protein structural study | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Chae, Pil Seok | - |
dc.identifier.doi | 10.1039/c5sc02900g | - |
dc.identifier.scopusid | 2-s2.0-84959449340 | - |
dc.identifier.wosid | 000371021900037 | - |
dc.identifier.bibliographicCitation | Chemical Science, v.7, no.3, pp.1933 - 1939 | - |
dc.relation.isPartOf | Chemical Science | - |
dc.citation.title | Chemical Science | - |
dc.citation.volume | 7 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 1933 | - |
dc.citation.endPage | 1939 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.subject.keywordPlus | GLYCOL GNG AMPHIPHILES | - |
dc.subject.keywordPlus | CRYSTAL-STRUCTURE | - |
dc.subject.keywordPlus | MNG AMPHIPHILES | - |
dc.subject.keywordPlus | SOLUBILIZATION | - |
dc.subject.keywordPlus | RECEPTOR | - |
dc.subject.keywordPlus | STABILIZATION | - |
dc.subject.keywordPlus | DETERGENTS | - |
dc.subject.keywordPlus | STABILITY | - |
dc.subject.keywordPlus | CRYSTALLIZATION | - |
dc.subject.keywordPlus | TRANSPORTER | - |
dc.identifier.url | https://pubs.rsc.org/en/content/articlelanding/2016/SC/C5SC02900G | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
55 Hanyangdeahak-ro, Sangnok-gu, Ansan, Gyeonggi-do, 15588, Korea+82-31-400-4269 sweetbrain@hanyang.ac.kr
COPYRIGHT © 2021 HANYANG UNIVERSITY. ALL RIGHTS RESERVED.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.