Preparative Isolation of Polar Antioxidant Constituents from Abies koreana Using Centrifugal Partition Chromatography Guided by DPPH center dot-HPLC Experiment

Abstract

Preparative separation of antioxidant constituents from the leaves of Abies koreana Wilson (Pinaceae) was performed by centrifugal partition chromatography (CPC) with a two-phase solvent system of ethyl acetate-isopropanol-water (9:1:10, v/v) target-guided by DPPH center dot-HPLC experiment. In DPPH center dot-HPLC experiment, trans-piceid exerted antioxidant activity, which was purified by Diaion-HP 20 macroporous resin chromatography and subsequent CPC. Maltol (98.3mg), dihydrokaempferol 7-O--D-glucopyranoside (230.4mg), and trans-piceid (165.9mg) were purified from 2.19g of crude extract with the purity over 95% by HPLC analysis. The chemical structures of isolated compounds were determined by H-1, C-13 NMR, and ESI-MS.