Photo-crosslinkable hydrogel-based 3D microfluidic culture device
DC Field | Value | Language |
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dc.contributor.author | Lee, Youlee | - |
dc.contributor.author | Lee, Jong Min | - |
dc.contributor.author | Bae, Pan-Kee | - |
dc.contributor.author | Chung, Il Yup | - |
dc.contributor.author | Chung, Bong Hyun | - |
dc.contributor.author | Chung, Bong Geun | - |
dc.date.accessioned | 2021-06-22T20:22:17Z | - |
dc.date.available | 2021-06-22T20:22:17Z | - |
dc.date.created | 2021-01-21 | - |
dc.date.issued | 2015-04 | - |
dc.identifier.issn | 0173-0835 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/18769 | - |
dc.description.abstract | We developed the photo-crosslinkable hydrogel-based 3D microfluidic device to culture neural stem cells (NSCs) and tumors. The photo-crosslinkable gelatin methacrylate (GelMA) polymer was used as a physical barrier in the microfluidic device and collagen type I gel was employed to culture NSCs in a 3D manner. We demonstrated that the pore size was inversely proportional to concentrations of GelMA hydrogels, showing the pore sizes of 5 and 25 w/v% GelMA hydrogels were 34 and 4 m, respectively. It also revealed that the morphology of pores in 5 w/v% GelMA hydrogels was elliptical shape, whereas we observed circular-shaped pores in 25 w/v% GelMA hydrogels. To culture NSCs and tumors in the 3D microfluidic device, we investigated the molecular diffusion properties across GelMA hydrogels, indicating that 25 w/v% GelMA hydrogels inhibited the molecular diffusion for 6 days in the 3D microfluidic device. In contrast, the chemicals were diffused in 5 w/v% GelMA hydrogels. Finally, we cultured NSCs and tumors in the hydrogel-based 3D microfluidic device, showing that 53-75% NSCs differentiated into neurons, while tumors were cultured in the collagen gels. Therefore, this photo-crosslinkable hydrogel-based 3D microfluidic culture device could be a potentially powerful tool for regenerative tissue engineering applications. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | John Wiley & Sons Ltd. | - |
dc.title | Photo-crosslinkable hydrogel-based 3D microfluidic culture device | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Chung, Il Yup | - |
dc.identifier.doi | 10.1002/elps.201400465 | - |
dc.identifier.scopusid | 2-s2.0-84927702482 | - |
dc.identifier.wosid | 000353052800004 | - |
dc.identifier.bibliographicCitation | Electrophoresis, v.36, no.7-8, pp.994 - 1001 | - |
dc.relation.isPartOf | Electrophoresis | - |
dc.citation.title | Electrophoresis | - |
dc.citation.volume | 36 | - |
dc.citation.number | 7-8 | - |
dc.citation.startPage | 994 | - |
dc.citation.endPage | 1001 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.subject.keywordPlus | NEURAL STEM-CELLS | - |
dc.subject.keywordPlus | DIFFERENTIATION | - |
dc.subject.keywordPlus | PLATFORM | - |
dc.subject.keywordPlus | COCULTURE | - |
dc.subject.keywordPlus | GROWTH | - |
dc.subject.keywordPlus | MODEL | - |
dc.subject.keywordAuthor | Hydrogel | - |
dc.subject.keywordAuthor | Microfluidic device | - |
dc.subject.keywordAuthor | Stem cell | - |
dc.identifier.url | https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/elps.201400465 | - |
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