A Src-family-tyrosine kinase, Lyn, is required for efficient IFN-beta, expression in pattern recognition receptor, RIG-I, signal pathway by interacting with IPS-1
DC Field | Value | Language |
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dc.contributor.author | Lim, Young Ju | - |
dc.contributor.author | Koo, Jung Bun | - |
dc.contributor.author | Hong, Eun-Hee | - |
dc.contributor.author | Park, Zee-Yong | - |
dc.contributor.author | Lim, Kyung-Min | - |
dc.contributor.author | Bae, Ok-Nam | - |
dc.contributor.author | Lee, Joo Young | - |
dc.date.accessioned | 2021-06-22T20:23:43Z | - |
dc.date.available | 2021-06-22T20:23:43Z | - |
dc.date.created | 2021-01-21 | - |
dc.date.issued | 2015-03 | - |
dc.identifier.issn | 1043-4666 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/18820 | - |
dc.description.abstract | Retinoic acid-inducible gene I (RIG-I) plays an important role in antiviral immunity as a cytosolic receptor recognizing invading viruses. The activation of downstream signaling pathways led by IFN-beta promoter stimulator-1 (IPS-1), an adaptor, is known to culminate in the activation of IRFs and the expression of type I interferons. However, the role of Src-family-tyrosine kinases (STKs) in the RIG-I signaling pathway has not been fully evaluated. Through a combined approach of immunoprecipitation and micro reversed phase liquid chromatography-tandem mass spectrometry (RPLC-MS/MS) analysis, we established that Lyn, one of the STKs, is associated with RIG-I in macrophages. The association of Lyn and RIG-I was confirmed by co-immunoprecipitation study with 293T cells overexpressing Lyn and RIG-I. Suppression of Lyn by siRNA knockdown or a pharmacological inhibitor (PP2) resulted in the attenuation of IRF3 activation and IFN-beta expression induced by short poly I:C, a RIG-I agonist, in macrophages. Lyn activation, as determined by phosphorylation of Tyr396 residue, was observed upon short poly I:C stimulation in the mitochondria of macrophages. Short poly I:C induced the formation of speckle-like aggregates of Lyn, which are prominent in mitochondria. Lyn associated with IPS-1, an adaptor protein of RIG-I, which resides on mitochondria membrane. Helicase domain of RIG-I and CARD of IPS-1 are responsible for the interaction with Lyn while SH3 and SH2 domains in Lyn are required for the association with RIG-I and IPS-1. Collectively, our results indicate that Lyn plays a positive regulatory role in RIG-I-mediated interferon expression as a downstream component of IPS-1. They provide further information as to how tyrosine kinases such as STKs play a role in the regulation of antiviral immunity. (C) 2014 Elsevier Ltd. All rights reserved. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD | - |
dc.title | A Src-family-tyrosine kinase, Lyn, is required for efficient IFN-beta, expression in pattern recognition receptor, RIG-I, signal pathway by interacting with IPS-1 | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Bae, Ok-Nam | - |
dc.identifier.doi | 10.1016/j.cyto.2014.12.008 | - |
dc.identifier.scopusid | 2-s2.0-84920895400 | - |
dc.identifier.wosid | 000351322400010 | - |
dc.identifier.bibliographicCitation | CYTOKINE, v.72, no.1, pp.63 - 70 | - |
dc.relation.isPartOf | CYTOKINE | - |
dc.citation.title | CYTOKINE | - |
dc.citation.volume | 72 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 63 | - |
dc.citation.endPage | 70 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalResearchArea | Immunology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Immunology | - |
dc.subject.keywordPlus | ADAPTER PROTEIN | - |
dc.subject.keywordPlus | VIRUS | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | INDUCTION | - |
dc.subject.keywordAuthor | Innate immunity | - |
dc.subject.keywordAuthor | Anti-viral signaling | - |
dc.subject.keywordAuthor | Src-family-tyrosine kinases | - |
dc.subject.keywordAuthor | IRF3 | - |
dc.subject.keywordAuthor | Type I interferon | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S104346661400622X?via%3Dihub | - |
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