Erythrophagocytosis of Lead-Exposed Erythrocytes by Renal Tubular Cells: Possible Role in Lead-Induced Nephrotoxicity
DC Field | Value | Language |
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dc.contributor.author | Kwon, So-Youn | - |
dc.contributor.author | Bae, Ok-Nam | - |
dc.contributor.author | Noh, Ji-Yoon | - |
dc.contributor.author | Kim, Keunyoung | - |
dc.contributor.author | Kang, Seojin | - |
dc.contributor.author | Shin, Young-Jun | - |
dc.contributor.author | Lim, Kyung-Min | - |
dc.contributor.author | Chung, Jin-Ho | - |
dc.date.accessioned | 2021-06-22T20:25:03Z | - |
dc.date.available | 2021-06-22T20:25:03Z | - |
dc.date.created | 2021-01-21 | - |
dc.date.issued | 2015-02 | - |
dc.identifier.issn | 0091-6765 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/18870 | - |
dc.description.abstract | BACKGROUND: Nephrotoxicity associated with lead poisoning has been frequently reported in epidemiological studies, but the underlying mechanisms have not been fully described. OBJECTIVES: We examined the role of erythrocytes, one of the major lead reservoirs, in lead-associated nephrotoxicity. METHODS AND RESULTS: Co-incubation of lead-exposed human erythrocytes with HK-2 human renal proximal tubular cells resulted in renal tubular cytotoxicity, suggesting a role of erythrocytes in lead-induced nephrotoxicity. Morphological and flow cytometric analyses revealed that HK-2 cells actively phagocytized lead-exposed erythrocytes, which was associated with phosphatidylserine (PS) externalization on the erythrocyte membrane and generation of PS-bearing microvesicles. Increased oxidative stress and up-regulation of nephrotoxic biomarkers, such as NGAL, were observed in HK-2 cells undergoing erythrophagocytosis. Moreover, TGF-beta, a marker of fibrosis, was also significantly up-regulated. We examined the significance of erythrophagocytosis in lead-induced nephrotoxicity in rats exposed to lead via drinking water for 12 weeks. We observed iron deposition and generation of oxidative stress in renal tissues of lead-exposed rats, as well as the histopathological alterations such as tubulointerstitial lesions, fibrosis, and up-regulation of KIM-1, NGAL, and TGF-beta. CONCLUSIONS: Our data strongly suggest that erythrophagocytosis and subsequent iron deposition in renal tubular cells could significantly enhance nephrotoxicity following lead exposure, providing insight on lead-associated kidney damages. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | US Department of Health and Human Services | - |
dc.title | Erythrophagocytosis of Lead-Exposed Erythrocytes by Renal Tubular Cells: Possible Role in Lead-Induced Nephrotoxicity | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Bae, Ok-Nam | - |
dc.identifier.doi | 10.1289/ehp.1408094 | - |
dc.identifier.scopusid | 2-s2.0-84923043716 | - |
dc.identifier.wosid | 000352079400011 | - |
dc.identifier.bibliographicCitation | Environmental Health Perspectives, v.123, no.2, pp.120 - 127 | - |
dc.relation.isPartOf | Environmental Health Perspectives | - |
dc.citation.title | Environmental Health Perspectives | - |
dc.citation.volume | 123 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 120 | - |
dc.citation.endPage | 127 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Environmental Sciences & Ecology | - |
dc.relation.journalResearchArea | Public, Environmental & Occupational Health | - |
dc.relation.journalResearchArea | Toxicology | - |
dc.relation.journalWebOfScienceCategory | Environmental Sciences | - |
dc.relation.journalWebOfScienceCategory | Public, Environmental & Occupational Health | - |
dc.relation.journalWebOfScienceCategory | Toxicology | - |
dc.subject.keywordPlus | CHRONIC KIDNEY-DISEASE | - |
dc.subject.keywordPlus | RAT-KIDNEY | - |
dc.subject.keywordPlus | PHOSPHATIDYLSERINE EXPOSURE | - |
dc.subject.keywordPlus | OXIDATIVE STRESS | - |
dc.subject.keywordPlus | BLOOD LEAD | - |
dc.subject.keywordPlus | INTERSTITIAL FIBROSIS | - |
dc.subject.keywordPlus | EPITHELIAL-CELLS | - |
dc.subject.keywordPlus | PROXIMAL TUBULE | - |
dc.subject.keywordPlus | UNITED-STATES | - |
dc.subject.keywordPlus | LOW-LEVEL | - |
dc.identifier.url | https://ehp.niehs.nih.gov/doi/10.1289/ehp.1408094 | - |
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