UV-B radiation-induced oxidative stress and p38 signaling pathway involvement in the benthic copepod Tigriopus japonicus
DC Field | Value | Language |
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dc.contributor.author | Kim, Bo-Mi | - |
dc.contributor.author | Rhee, Jae-Sung | - |
dc.contributor.author | Lee, Kyun-Woo | - |
dc.contributor.author | Kim, Min-Jung | - |
dc.contributor.author | Shin, Kyung-Hoon | - |
dc.contributor.author | Lee, Su-Jae | - |
dc.contributor.author | Lee, Young-Mi | - |
dc.contributor.author | Lee, Jae-Seong | - |
dc.date.accessioned | 2021-06-22T20:42:57Z | - |
dc.date.available | 2021-06-22T20:42:57Z | - |
dc.date.created | 2021-01-21 | - |
dc.date.issued | 2015-01 | - |
dc.identifier.issn | 1532-0456 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/19254 | - |
dc.description.abstract | Ultraviolet B (UV-B) radiation presents an environmental hazard to aquatic organisms. To understand the molecular responses of the intertidal copepod Tigriopus japonicus to UV-B radiation, we measured the acute toxicity response to 96 h of UV-B radiation, and we also assessed the intracellular reactive oxygen species (ROS) levels, glutathione (GSH) content, and antioxidant enzyme (GST, GR, GPx, and SOD) activities after 24 h of exposure to UV-B with LD50 and half LD50 values. Also, expression patterns of p53 and hsp gene families with phosphorylation of p38 MAPK were investigated in UV-B-exposed copepods. We found that the ROS level, GSH content, and antioxidant enzyme activity levels were increased with the transcriptional upregulation of antioxidant-related genes, indicating that UV-B induces oxidative stress by generating ROS and stimulating antioxidant enzymatic activity as a defense mechanism. Additionally, we found that p53 expression was significantly increased after UV-B irradiation due to increases in the phosphorylation of the stress-responsive p38 MAPK, indicating that UV-B may be responsible for inducing DNA damage in T. japonicus. Of the hsp family genes, transcriptional levels of hsp20, hsp20.7, hsp70, and hsp90 were elevated in response to a low dose of UV-B radiation (9 kJ m(-2)), suggesting that these hsp genes may be involved in cellular protection against UV-B radiation. In this paper, we performed a pathway-oriented mechanistic analysis in response to UV-B radiation, and this analysis provides a better understanding of the effects of UV-B in the intertidal benthic copepod T. japonicus. (C) 2014 Elsevier Inc. All rights reserved. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | Elsevier BV | - |
dc.title | UV-B radiation-induced oxidative stress and p38 signaling pathway involvement in the benthic copepod Tigriopus japonicus | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Shin, Kyung-Hoon | - |
dc.identifier.doi | 10.1016/j.cbpc.2014.08.003 | - |
dc.identifier.scopusid | 2-s2.0-84907344039 | - |
dc.identifier.wosid | 000348825300003 | - |
dc.identifier.bibliographicCitation | Comparative Biochemistry and Physiology, Part C, v.167, pp.15 - 23 | - |
dc.relation.isPartOf | Comparative Biochemistry and Physiology, Part C | - |
dc.citation.title | Comparative Biochemistry and Physiology, Part C | - |
dc.citation.volume | 167 | - |
dc.citation.startPage | 15 | - |
dc.citation.endPage | 23 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Endocrinology & Metabolism | - |
dc.relation.journalResearchArea | Toxicology | - |
dc.relation.journalResearchArea | Zoology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Endocrinology & Metabolism | - |
dc.relation.journalWebOfScienceCategory | Toxicology | - |
dc.relation.journalWebOfScienceCategory | Zoology | - |
dc.subject.keywordPlus | HEAT-SHOCK PROTEINS | - |
dc.subject.keywordPlus | INDUCED DNA-DAMAGE | - |
dc.subject.keywordPlus | ULTRAVIOLET-RADIATION | - |
dc.subject.keywordPlus | INTERTIDAL COPEPOD | - |
dc.subject.keywordPlus | SUPEROXIDE-DISMUTASE | - |
dc.subject.keywordPlus | MOLECULAR CHAPERONES | - |
dc.subject.keywordPlus | LIPID-PEROXIDATION | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | KINASE | - |
dc.subject.keywordAuthor | Tigriopus japonicus | - |
dc.subject.keywordAuthor | Copepod | - |
dc.subject.keywordAuthor | UV-B | - |
dc.subject.keywordAuthor | Oxidative stress | - |
dc.subject.keywordAuthor | p38 MAPK | - |
dc.subject.keywordAuthor | p53 | - |
dc.subject.keywordAuthor | Heat shock protein | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S1532045614001082?via%3Dihub | - |
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