HY253, a Novel Decahydrofluorene Analog, Induces Apoptosis via Intrinsic Pathway and Cell Cycle Arrest in Liver Cancer HepG2 Cells
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 최고운 | - |
dc.contributor.author | 서혜원 | - |
dc.contributor.author | 장승훈 | - |
dc.contributor.author | 김동식 | - |
dc.contributor.author | 이철훈 | - |
dc.date.accessioned | 2021-06-22T21:23:20Z | - |
dc.date.available | 2021-06-22T21:23:20Z | - |
dc.date.issued | 2015-03 | - |
dc.identifier.issn | 1017-7825 | - |
dc.identifier.issn | 1738-8872 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/20170 | - |
dc.description.abstract | Recently, we isolated HY253, a novel decahydrofluorene analog with a molecular structure of 7,8a-divinyl-2,4a,4b,5,6,7,8,8a,9,9a-decahydro-1H-fluorene-2,4a,4b,9a-tetraol from the roots of Aralia continentalis, which is known as Dokwhal (獨活), a traditional medicinal herb. Moreover, we previously reported its cytotoxic activity on cancer cell proliferation in human lung cancer A549 and cervical cancer HeLa cells. The current study aimed to evaluate its detailed molecular mechanisms in cell cycle arrest and apoptotic induction in human hepatocellular carcinoma HepG2 cells. Flow cytometric analysis of HepG2 cells treated with 60 μM HY253 revealed appreciable cell cycle arrest at the G1 phase via inhibition of Rb phosphorylation and down-regulation of cyclin D1. Furthermore, using western blots, we found that up-regulation of cyclin-dependent kinase inhibitors, such as p21CIP1 and p27KIP1, was associated with this G1 phase arrest. Moreover, TUNEL assay and immunoblottings revealed apoptotic induction in HepG2 cells treated with 100 μM HY253 for 24 h, which is associated with cytochrome c release from mitochondria, via down-regulation of anti-apoptotic Bcl-2 protein, which in turn resulted in activation of caspase-9 and -3, and proteolytic cleavage of poly(ADP-ribose) polymerase (PARP). Accordingly, we suggest that HY253 may be a potent chemotherapeutic hit compound for treating human liver cancer cells via up-regulation and activation of the p53 gene. | - |
dc.format.extent | 5 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | 한국미생물·생명공학회 | - |
dc.title | HY253, a Novel Decahydrofluorene Analog, Induces Apoptosis via Intrinsic Pathway and Cell Cycle Arrest in Liver Cancer HepG2 Cells | - |
dc.type | Article | - |
dc.publisher.location | 대한민국 | - |
dc.identifier.doi | 10.4014/jmb.1501.01069 | - |
dc.identifier.scopusid | 2-s2.0-84925358892 | - |
dc.identifier.wosid | 000351975800016 | - |
dc.identifier.bibliographicCitation | Journal of Microbiology and Biotechnology, v.25, no.3, pp 413 - 417 | - |
dc.citation.title | Journal of Microbiology and Biotechnology | - |
dc.citation.volume | 25 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 413 | - |
dc.citation.endPage | 417 | - |
dc.identifier.kciid | ART001972726 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.subject.keywordPlus | DEPENDENT KINASE INHIBITOR | - |
dc.subject.keywordPlus | CYTOCHROME-C | - |
dc.subject.keywordPlus | LEUKEMIA-CELLS | - |
dc.subject.keywordPlus | INDUCTION | - |
dc.subject.keywordPlus | CLEAVAGE | - |
dc.subject.keywordPlus | RELEASE | - |
dc.subject.keywordPlus | MCS-C2 | - |
dc.subject.keywordPlus | AGENT | - |
dc.subject.keywordAuthor | Aralia continentalis | - |
dc.subject.keywordAuthor | apoptosis | - |
dc.subject.keywordAuthor | p27KIP1 | - |
dc.subject.keywordAuthor | p21CIP1 | - |
dc.subject.keywordAuthor | cytochrome c | - |
dc.subject.keywordAuthor | HepG2 | - |
dc.identifier.url | https://www.jmb.or.kr/journal/view.html?doi=10.4014/jmb.1501.01069 | - |
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