CR389, a Benzoimidazolyl Pyridinone Analog, Induces Cell Cycle Arrest and Apoptosis via p53 Activation in Human Ovarian Cancer PA-1 Cells
DC Field | Value | Language |
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dc.contributor.author | 서혜원 | - |
dc.contributor.author | 최고운 | - |
dc.contributor.author | 이철훈 | - |
dc.date.accessioned | 2021-06-22T21:23:23Z | - |
dc.date.available | 2021-06-22T21:23:23Z | - |
dc.date.created | 2021-01-22 | - |
dc.date.issued | 2015-03 | - |
dc.identifier.issn | 1017-7825 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/20172 | - |
dc.description.abstract | In the course of screening for novel cell cycle inhibitors and apoptotic inducers, CR389, elucidated as 5-(1H-benzoimidazol-2-yl)-1H-pyridin-2-one, was generated as a new hit compound. Flow cytometric analysis and western blots of PA-1 cells treated with 40 μM CR389 revealed an appreciable cell cycle arrest at the G2/M phase through direct inhibition of the CDK1 complex. In addition, activation of p53 via phosphorylation at Ser15 and subsequent up-regulation of p21CIP1 showed that CR389 also induces p53-dependent-p21CIP1-mediated cell cycle arrest. Furthermore, apoptotic induction in 40 μM CR389-treated PA-1 cells is associated with the release of cytochrome c from mitochondria through up-regulation of the proapoptotic Bax protein, which results in the activation of procaspase-9 and -3, and the cleavage of poly(ADP-ribose) polymerase (PARP). Accordingly, CR389 seems to have multiple mechanisms of antiproliferative activity through p53-mediated pathways against human ovarian cancer cells. Therefore, we conclude that CR389 is a candidate therapeutic agent for the treatment of human ovarian cancer via the activation of p53. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | 한국미생물·생명공학회 | - |
dc.title | CR389, a Benzoimidazolyl Pyridinone Analog, Induces Cell Cycle Arrest and Apoptosis via p53 Activation in Human Ovarian Cancer PA-1 Cells | - |
dc.title.alternative | CR389, a Benzoimidazolyl Pyridinone Analog, Induces Cell Cycle Arrest and Apoptosis via p53 Activation in Human Ovarian Cancer PA-1 Cells | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | 이철훈 | - |
dc.identifier.doi | 10.4014/jmb.1412.12080 | - |
dc.identifier.scopusid | 2-s2.0-84925355153 | - |
dc.identifier.wosid | 000351975800017 | - |
dc.identifier.bibliographicCitation | Journal of Microbiology and Biotechnology, v.25, no.3, pp.418 - 422 | - |
dc.relation.isPartOf | Journal of Microbiology and Biotechnology | - |
dc.citation.title | Journal of Microbiology and Biotechnology | - |
dc.citation.volume | 25 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 418 | - |
dc.citation.endPage | 422 | - |
dc.type.rims | ART | - |
dc.identifier.kciid | ART001972727 | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.subject.keywordAuthor | p53 | - |
dc.subject.keywordAuthor | p21CIP1 | - |
dc.subject.keywordAuthor | Bax | - |
dc.subject.keywordAuthor | apoptotic induction | - |
dc.subject.keywordAuthor | cytochrome c | - |
dc.subject.keywordAuthor | PA-1 cells | - |
dc.identifier.url | https://www.jmb.or.kr/journal/view.html?doi=10.4014/jmb.1412.12080 | - |
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