Enhanced Detection of Infectious Pancreatic Necrosis Virus via Lateral Flow Chip and Fluorometric Biosensors Based on Self-Assembled Protein Nanoprobes
DC Field | Value | Language |
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dc.contributor.author | Chayan, Sachin G. | - |
dc.contributor.author | Kim, Dasom | - |
dc.contributor.author | Hwang, Jangsun | - |
dc.contributor.author | Choi, Yonghyun | - |
dc.contributor.author | Hong, Jong Wook | - |
dc.contributor.author | Kim, Jeongho | - |
dc.contributor.author | Lee, Min-Ho | - |
dc.contributor.author | Hwang, Mintai P. | - |
dc.contributor.author | Choi, Jonghoon | - |
dc.date.accessioned | 2021-06-22T09:25:46Z | - |
dc.date.available | 2021-06-22T09:25:46Z | - |
dc.date.created | 2021-01-21 | - |
dc.date.issued | 2019-11 | - |
dc.identifier.issn | 2379-3694 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/2064 | - |
dc.description.abstract | Salmon fish farmers face remarkable problems in fish rearing and handling due to the spread of disease by infectious pancreatic necrosis virus (IPNV). Therefore, we developed a straightforward and sensitive technique to detect IPNV-based on recombinant human apoferritin heavy chain (hAFN-H) protein nanoparticles. In this study, the 24 subunits of the hAFN-H were genetically modified to express 6xHis-tag and protein-G at their C-terminal site using Escherichia coli. We thus achieved a two-step signal amplifying strategy that utilizes a recombinant hAFN-H nanoprobe having a protein-G-binding site that targets the F-c region of monoclonal antibodies and a 6xHis-tag that actively interacts with the functionalized Ni-NTA derivatives. In this study, we report a considerable advancement in magnetic bead-based detection systems that use Ni-NTA-Atto 550, reliably exhibiting detection limits of 1.02 TCID50/mL (50% tissue culture infective dose). Additionally, we propose a lateral flow chip-based detection method that uses the hAFN-H surface functionalized with 5 nm of the Ni-NTA-nanogold complex as a nanoprobe; the limit of detection towards IPNV was 0.88 TCID50/mL. The detection of IPNV by this recombinant hAFN-H nanoprobe was linear to virus titers in the range of 10(1)-10(3) TCID50/mL. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.title | Enhanced Detection of Infectious Pancreatic Necrosis Virus via Lateral Flow Chip and Fluorometric Biosensors Based on Self-Assembled Protein Nanoprobes | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Hong, Jong Wook | - |
dc.identifier.doi | 10.1021/acssensors.9b01362 | - |
dc.identifier.scopusid | 2-s2.0-85074393677 | - |
dc.identifier.wosid | 000499737100015 | - |
dc.identifier.bibliographicCitation | ACS SENSORS, v.4, no.11, pp.2937 - 2944 | - |
dc.relation.isPartOf | ACS SENSORS | - |
dc.citation.title | ACS SENSORS | - |
dc.citation.volume | 4 | - |
dc.citation.number | 11 | - |
dc.citation.startPage | 2937 | - |
dc.citation.endPage | 2944 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.subject.keywordPlus | IGG-BINDING | - |
dc.subject.keywordPlus | IPNV | - |
dc.subject.keywordPlus | POLYMERASE | - |
dc.subject.keywordPlus | PLATFORM | - |
dc.subject.keywordPlus | ASSAY | - |
dc.subject.keywordPlus | IMMUNOASSAY | - |
dc.subject.keywordPlus | LEUKOCYTES | - |
dc.subject.keywordPlus | LETHAL | - |
dc.subject.keywordPlus | LIMIT | - |
dc.subject.keywordAuthor | magnetic bead complex | - |
dc.subject.keywordAuthor | infectious pancreatic necrosis virus | - |
dc.subject.keywordAuthor | human apoferritin nanoprobe | - |
dc.subject.keywordAuthor | paper-based immunoassay | - |
dc.subject.keywordAuthor | Ni-NTA gold nanoparticle | - |
dc.identifier.url | https://pubs.acs.org/doi/10.1021/acssensors.9b01362 | - |
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