Effects of Intestinal Microbiota on the Bioavailability of Geniposide in Rats

Abstract

This study investigated the effects of intestinal microbiota on the metabolism of geniposide by using a rat model treated with a mixture of antibiotics. The plasma concentration of geniposide was determined after oral administration in control and antibiotics treated rates by using liquid chromatography-tandem mass spectrometry. The maximum plasma concentration (C-max) of geniposide in control and antibiotics-treated rats were 0.91 +/- 0.26 and 1.01 +/- 0.04 mu g/mL, respectively, and the area under the curve (AUC) values were 7.34 +/- 3.32 and 11.9 +/- 2.1 mu g.h/mL (p < 0.05), respectively. The levels of geniposide in rat feces were 0.64 and 15.6 mg, respectively, in the control and antibiotics-treated groups. thus. the systemic exposure of geniposide was greater in the antibiotics-treated rats. This may be due to the antibiotic induced suppression of the metabolic activities of the intestinal microbiota. These results suggest that the gut microbiota may have an impact on the bioavialability of geniposide