Synergistic effect of simvastatin plus NS398 on inhibition of proliferation and survival in hepatocellular carcinoma cell line
DC Field | Value | Language |
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dc.contributor.author | Lee, Sun Jae | - |
dc.contributor.author | Hwang, Ji Won | - |
dc.contributor.author | Yim, Hyungshin | - |
dc.contributor.author | Yim, Hyung Joon | - |
dc.contributor.author | Woo, Sang Uk | - |
dc.contributor.author | Suh, Sang Jun | - |
dc.contributor.author | Hyun, Jong Jin | - |
dc.contributor.author | Jung, Sung Woo | - |
dc.contributor.author | Koo, Ja Seol | - |
dc.contributor.author | Kim, Ji Hoon | - |
dc.contributor.author | Seo, Yeon Seok | - |
dc.contributor.author | Yeon, Jong Eun | - |
dc.contributor.author | Lee, Sang Woo | - |
dc.contributor.author | Byun, Kwan Soo | - |
dc.contributor.author | Um, Soon Ho | - |
dc.date.accessioned | 2021-06-22T23:23:20Z | - |
dc.date.available | 2021-06-22T23:23:20Z | - |
dc.date.created | 2021-01-21 | - |
dc.date.issued | 2014-06 | - |
dc.identifier.issn | 0815-9319 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/22840 | - |
dc.description.abstract | Background and AimsNS398, a selective cyclooxygenase-2 inhibitor, and simvastatin, a 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitor, both exert an anticancer effect on hepatocellular carcinoma cells, but the effect of co-administration of the two drugs remains unknown. We aimed to investigate the synergistic in vitro anticancer effect of co-administration of NS398 and simvastatin and its mechanism. MethodsThe Hep3B and Huh-7 cell lines were cultured. Cells were treated with simvastatin, NS398, or a combination. 5-bromo-2-deoxyuridine ELISA assay, flow cytometry, Western blot analyses, and immunofluorescence assay were performed. ResultsIn both cell lines, co-administration of simvastatin and NS398 resulted in a greater effect on proliferation and apoptosis. In Hep3B cells, co-administration of the two drugs resulted in a greater decrease in procaspase 3 and Bcl-2 and an increase in cleaved caspase 9 than that noted with monotherapy. In Huh-7 cells, co-administration of the two drugs resulted in a greater decrease in procaspase 3 and cyclin D1 and an increase in cleaved caspase 9. Expression of NF-B and Akt were also decreased to a greater extent when the two drugs were co-administered in both cell lines. Immunofluorescence assay showed suppression of the nuclear localization of NF-B by simvastatin or NS398. The effect was greater by co-administration. ConclusionsThe co-administration of NS398 and simvastatin produced greater antiproliferative and proapoptotic effects against Hep3B cells and Huh-7 cells. Inhibition of the NF-B and Akt pathway and activation of caspase cascade, which are considered as the major mechanism of synergistic anticancer properties, were observed in both cell lines. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | WILEY | - |
dc.title | Synergistic effect of simvastatin plus NS398 on inhibition of proliferation and survival in hepatocellular carcinoma cell line | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Yim, Hyungshin | - |
dc.identifier.doi | 10.1111/jgh.12503 | - |
dc.identifier.scopusid | 2-s2.0-84900563613 | - |
dc.identifier.wosid | 000335973300028 | - |
dc.identifier.bibliographicCitation | JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY, v.29, no.6, pp.1299 - 1307 | - |
dc.relation.isPartOf | JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY | - |
dc.citation.title | JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY | - |
dc.citation.volume | 29 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 1299 | - |
dc.citation.endPage | 1307 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Gastroenterology & Hepatology | - |
dc.relation.journalWebOfScienceCategory | Gastroenterology & Hepatology | - |
dc.subject.keywordPlus | NF-KAPPA-B | - |
dc.subject.keywordPlus | INDUCED GROWTH-INHIBITION | - |
dc.subject.keywordPlus | COLON-CANCER CELLS | - |
dc.subject.keywordPlus | ANTICANCER THERAPY | - |
dc.subject.keywordPlus | MEVALONATE PATHWAY | - |
dc.subject.keywordPlus | DOWN-REGULATION | - |
dc.subject.keywordPlus | LIVER-CANCER | - |
dc.subject.keywordPlus | APOPTOSIS | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | CELECOXIB | - |
dc.subject.keywordAuthor | combination drug therapy | - |
dc.subject.keywordAuthor | hepatocellular carcinoma | - |
dc.subject.keywordAuthor | cyclooxygenase-2 inhibitor | - |
dc.subject.keywordAuthor | simvastatin | - |
dc.identifier.url | https://onlinelibrary.wiley.com/doi/10.1111/jgh.12503 | - |
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