Basal-type lumenogenesis in extraembryonic endoderm stem cells models the early visceral endoderm
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Minjae | - |
dc.contributor.author | Zhong, Yixiang | - |
dc.contributor.author | Jung, Kyoung Hwa | - |
dc.contributor.author | Chai, Young Gyu | - |
dc.contributor.author | Binas, Bert | - |
dc.date.accessioned | 2021-06-22T09:41:59Z | - |
dc.date.available | 2021-06-22T09:41:59Z | - |
dc.date.issued | 2019-10 | - |
dc.identifier.issn | 0021-9533 | - |
dc.identifier.issn | 1477-9137 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/2306 | - |
dc.description.abstract | Cultured rat primitive extraembryonic endoderm (pXEN) cells easily form free-floating multicellular vesicles de novo, exemplifying a poorly studied type of morphogenesis. Here, we reveal the underlying mechanism and the identity of the vesicles. We resolve the morphogenesis into vacuolization, vesiculation and maturation, and define the molecular characteristics and requirements of each step. Vacuolization is fueled by macropinocytosis and occurs by default if not blocked by high cell density or matrix proteins. Fine-tuned cell-cell contact then forms nascent three-cell vesicles with vacuolederived lumina. In maturation, the vesicles complete epithelialization, expand viamitosis and continued fluid uptake, and differentiate further. The mature vesicles consist of a simple squamous epithelium with an apical-outside/basal-inside polarity that we trace back to the single cell stage. The polarity and gene expression pattern of the vesicles are similar to those of the early visceral endoderm. pXEN cells provide a useful in vitro model for study of matrix-independent, basal-type lumenogenesis and the physiology of the visceral endoderm. | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | COMPANY BIOLOGISTS LTD | - |
dc.title | Basal-type lumenogenesis in extraembryonic endoderm stem cells models the early visceral endoderm | - |
dc.type | Article | - |
dc.publisher.location | 영국 | - |
dc.identifier.doi | 10.1242/jcs.230607 | - |
dc.identifier.scopusid | 2-s2.0-85073125129 | - |
dc.identifier.wosid | 000490938000008 | - |
dc.identifier.bibliographicCitation | JOURNAL OF CELL SCIENCE, v.132, no.19 | - |
dc.citation.title | JOURNAL OF CELL SCIENCE | - |
dc.citation.volume | 132 | - |
dc.citation.number | 19 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.subject.keywordPlus | MOUSE EMBRYOS | - |
dc.subject.keywordPlus | XEN CELLS | - |
dc.subject.keywordPlus | LUMEN | - |
dc.subject.keywordPlus | MORPHOGENESIS | - |
dc.subject.keywordPlus | ORIENTATION | - |
dc.subject.keywordPlus | POLARITY | - |
dc.subject.keywordPlus | LINE | - |
dc.subject.keywordPlus | DIFFERENTIATION | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | RECEPTORS | - |
dc.subject.keywordAuthor | Stem cells | - |
dc.subject.keywordAuthor | Visceral endoderm | - |
dc.subject.keywordAuthor | Vacuole | - |
dc.subject.keywordAuthor | Lumen | - |
dc.subject.keywordAuthor | Vesicle | - |
dc.subject.keywordAuthor | Polarity | - |
dc.identifier.url | https://journals.biologists.com/jcs/article/132/19/jcs230607/223896/Basal-type-lumenogenesis-in-extraembryonic | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
55 Hanyangdeahak-ro, Sangnok-gu, Ansan, Gyeonggi-do, 15588, Korea+82-31-400-4269 sweetbrain@hanyang.ac.kr
COPYRIGHT © 2021 HANYANG UNIVERSITY. ALL RIGHTS RESERVED.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.