Hydrophobic Variations of N-Oxide Amphiphiles for Membrane Protein Manipulation: Importance of Non-hydrocarbon Groups in the Hydrophobic Portion
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Chae, Pil Seok | - |
dc.contributor.author | Sadaf, Aiman | - |
dc.contributor.author | Gellman, Samuel H. | - |
dc.date.accessioned | 2021-06-23T00:22:58Z | - |
dc.date.available | 2021-06-23T00:22:58Z | - |
dc.date.created | 2021-01-21 | - |
dc.date.issued | 2014-01 | - |
dc.identifier.issn | 1861-4728 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/24123 | - |
dc.description.abstract | Amphipathic agents called detergents serve as membrane-mimetic systems to maintain the native structures of membrane proteins during their manipulation. However, membrane proteins solubilized in conventional detergents tend to undergo denaturation and aggregation, necessitating the development of novel amphipathic agents with enhanced properties. Here we describe several new amphiphiles that contain an N-oxide group as the hydrophilic portion. The new amphiphiles have been evaluated for the ability to solubilize and stabilize a fragile multi-subunit assembly from biological membranes. We found that cholate-based agents were promising in supporting retention of the native protein quaternary structure, while deoxycholate-based amphiphiles were highly efficient in extracting/solubilizing the intact superassembly from the native membrane. Monitoring superassembly solubilization and stabilization as a function of variation in amphiphile structure led us to propose that a non-hydrocarbon moiety such as an amide, ether, or a hydroxy group present in the lipophilic regions can manifest distinctive effects in the context of membrane protein manipulation. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | Wiley - V C H Verlag GmbbH & Co. | - |
dc.title | Hydrophobic Variations of N-Oxide Amphiphiles for Membrane Protein Manipulation: Importance of Non-hydrocarbon Groups in the Hydrophobic Portion | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Chae, Pil Seok | - |
dc.identifier.doi | 10.1002/asia.201301097 | - |
dc.identifier.scopusid | 2-s2.0-84890788321 | - |
dc.identifier.wosid | 000328501400016 | - |
dc.identifier.bibliographicCitation | Chemistry - An Asian Journal, v.9, no.1, pp.110 - 116 | - |
dc.relation.isPartOf | Chemistry - An Asian Journal | - |
dc.citation.title | Chemistry - An Asian Journal | - |
dc.citation.volume | 9 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 110 | - |
dc.citation.endPage | 116 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.subject.keywordPlus | MUSCARINIC ACETYLCHOLINE-RECEPTOR | - |
dc.subject.keywordPlus | BETA(2) ADRENERGIC-RECEPTOR | - |
dc.subject.keywordPlus | FACIAL AMPHIPHILES | - |
dc.subject.keywordPlus | AQUEOUS-SOLUTIONS | - |
dc.subject.keywordPlus | CRYSTAL-STRUCTURE | - |
dc.subject.keywordPlus | MNG AMPHIPHILES | - |
dc.subject.keywordPlus | STABILIZATION | - |
dc.subject.keywordPlus | DETERGENT | - |
dc.subject.keywordPlus | SOLUBILIZATION | - |
dc.subject.keywordPlus | CRYSTALLIZATION | - |
dc.subject.keywordAuthor | amphiphiles | - |
dc.subject.keywordAuthor | membrane proteins | - |
dc.subject.keywordAuthor | molecular design | - |
dc.subject.keywordAuthor | non-hydrocarbon groups | - |
dc.subject.keywordAuthor | stabilization | - |
dc.identifier.url | https://onlinelibrary.wiley.com/doi/full/10.1002/asia.201301097 | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
55 Hanyangdeahak-ro, Sangnok-gu, Ansan, Gyeonggi-do, 15588, Korea+82-31-400-4269 sweetbrain@hanyang.ac.kr
COPYRIGHT © 2021 HANYANG UNIVERSITY. ALL RIGHTS RESERVED.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.