Synthesis, Characterization, and Directional Binding of Anisotropic Biohybrid Microparticles for Multiplexed Biosensing
DC Field | Value | Language |
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dc.contributor.author | Jung, Chan Woo | - |
dc.contributor.author | Jalani, Ghulam | - |
dc.contributor.author | Ko, Juhui | - |
dc.contributor.author | Choo, Jaebum | - |
dc.contributor.author | Lim, Dong Woo | - |
dc.date.accessioned | 2021-06-23T00:23:17Z | - |
dc.date.available | 2021-06-23T00:23:17Z | - |
dc.date.created | 2021-01-21 | - |
dc.date.issued | 2014-01 | - |
dc.identifier.issn | 1022-1336 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/24134 | - |
dc.description.abstract | Anisotropic microarchitectures with different physicochemical properties have been developed as advanced materials for challenging industrial and biomedical applications including switchable displays, multiplexed biosensors and bioassays, spatially-controlled drug delivery systems, and tissue engineering scaffolds. In this study, anisotropic biohybrid microparticles (MPs) spatio-selectively conjugated with two different antibodies (Abs) are first developed for fluorescence-based, multiplexed sensing of biological molecules. Poly(acrylamide-co-acrylic acid) is chemically modified with maleimide- or acetylene groups to introduce different targeting biological moieties into each compartment of anisotropic MPs. Modified polymer solutions containing two different fluorescent dyes are separately used for electrohydrodynamic co-jetting with side-by-side needle geometry. The anisotropic MPs are chemically stabilized by thermal imidization, followed by bioconjugation of two different sets of polyclonal Abs with two individual compartments via maleimide-thiol coupling reaction and Huisgen 1,3-dipolar cycloaddition. Finally, two compartments of the anisotropic biohybrid MPs are spatio-selectively associated with the respective monoclonal Ab-immobilized substrate in the presence of the antigen by sandwich-type immunocomplex formation, resulting in their ordered orientation due to the spatio-specific molecular interaction, as confirmed by confocal laser scanning microscopy. In conclusion, anisotropic biohybrid MPs capable of directional binding have great potential as a new fluorescence-based multiplexing biosensing system. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | WILEY-V C H VERLAG GMBH | - |
dc.title | Synthesis, Characterization, and Directional Binding of Anisotropic Biohybrid Microparticles for Multiplexed Biosensing | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lim, Dong Woo | - |
dc.identifier.doi | 10.1002/marc.201300652 | - |
dc.identifier.scopusid | 2-s2.0-84891627766 | - |
dc.identifier.wosid | 000336481700006 | - |
dc.identifier.bibliographicCitation | MACROMOLECULAR RAPID COMMUNICATIONS, v.35, no.1, pp.56 - 65 | - |
dc.relation.isPartOf | MACROMOLECULAR RAPID COMMUNICATIONS | - |
dc.citation.title | MACROMOLECULAR RAPID COMMUNICATIONS | - |
dc.citation.volume | 35 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 56 | - |
dc.citation.endPage | 65 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Polymer Science | - |
dc.relation.journalWebOfScienceCategory | Polymer Science | - |
dc.subject.keywordPlus | JANUS PARTICLES | - |
dc.subject.keywordPlus | COLLOIDAL PARTICLES | - |
dc.subject.keywordPlus | PICKERING EMULSION | - |
dc.subject.keywordPlus | CLICK CHEMISTRY | - |
dc.subject.keywordPlus | NANOPARTICLES | - |
dc.subject.keywordPlus | PROBES | - |
dc.subject.keywordPlus | FUNCTIONALIZATION | - |
dc.subject.keywordPlus | IMMUNOASSAY | - |
dc.subject.keywordPlus | DIAGNOSTICS | - |
dc.subject.keywordPlus | FABRICATION | - |
dc.subject.keywordAuthor | anisotropy | - |
dc.subject.keywordAuthor | biohybrid microparticles | - |
dc.subject.keywordAuthor | electrohydrodynamic co-jetting | - |
dc.subject.keywordAuthor | multiplexed biosensing | - |
dc.subject.keywordAuthor | sandwich immunocomplex | - |
dc.identifier.url | https://onlinelibrary.wiley.com/doi/10.1002/marc.201300652 | - |
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