Enzymatic Fractionation of SAA-Pretreated Barley Straw for Production of Fuel Ethanol and Astaxanthin as a Value-Added Co-Product
- Authors
- Nghiem, Nhuan P.; Kim, Tae Hyun; Yoo, Chang Geun; Hicks, Kevin B.
- Issue Date
- Sep-2013
- Publisher
- SPRINGER
- Keywords
- Biomass bioconversion; Biorefinery; Integrated process; Fuel ethanol; Value-added product; Simultaneous saccharification and fermentation
- Citation
- APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, v.171, no.2, pp.341 - 351
- Indexed
- SCIE
SCOPUS
- Journal Title
- APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
- Volume
- 171
- Number
- 2
- Start Page
- 341
- End Page
- 351
- URI
- https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/27138
- DOI
- 10.1007/s12010-013-0374-0
- ISSN
- 0273-2289
- Abstract
- Barley straw was used to demonstrate an integrated process for production of fuel ethanol and astaxanthin as a value-added co-product. Barley straw was pretreated by soaking in aqueous ammonia using the previously determined optimum conditions, which included 77.6 A degrees C treatment temperature, 12.1 h treatment time, 15 wt% ammonia concentration, and 1:8 solid-to-liquid ratio. In the newly developed process, the pretreated barley straw was first hydrolyzed with ACCELLERASEA (R) XY (a commercial hemicellulase product) to generate a xylose-rich solution, which contained 3.8 g/l glucose, 22.9 g/l xylose, and 2.4 g/l arabinose, with 96 % of the original glucan being left intact. The xylose-rich solution was used for production of astaxanthin by the yeast Phaffia rhodozyma without further treatment. The resulting cellulose-enriched solid residue was used for ethanol production in a fed-batch simultaneous saccharification and fermentation using ACCELLERASEA (R) 1500 (a commercial cellulase product) and the industrial yeast Saccharomyces cerevisiae. At the end of the fermentation, 70 g/l ethanol was obtained, which was equivalent to 63 % theoretical yield based on the glucan content of the solid substrate.
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