Tributyltin Affects Retinoid X Receptor-Mediated Lipid Metabolism in the Marine Rotifer Brachionus koreanus
DC Field | Value | Language |
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dc.contributor.author | Lee, Min-Chul | - |
dc.contributor.author | Fonseca, Elza | - |
dc.contributor.author | Park, Jun Chul | - |
dc.contributor.author | Yoon, Deok-Seo | - |
dc.contributor.author | Choi, Hyuntae | - |
dc.contributor.author | Kim, Moonkoo | - |
dc.contributor.author | Han, Jeonghoon | - |
dc.contributor.author | Cho, Hyeon-Seo | - |
dc.contributor.author | Shin, Kyung-Hoon | - |
dc.contributor.author | Santos, Miguel L. | - |
dc.contributor.author | Jung, Jee-Hyun | - |
dc.contributor.author | Castro, L. Filipe C. | - |
dc.contributor.author | Lee, Jae-Seong | - |
dc.date.accessioned | 2021-06-22T10:00:55Z | - |
dc.date.available | 2021-06-22T10:00:55Z | - |
dc.date.created | 2021-01-21 | - |
dc.date.issued | 2019-07 | - |
dc.identifier.issn | 0013-936X | - |
dc.identifier.uri | https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/2760 | - |
dc.description.abstract | To examine how tributyltin (TBT), a model obesogen, affects the lipid metabolism in the marine rotifer Brachionus koreanus, we carried out life-cycle studies and determined the in vitro and in silico interactions of retinoid X receptor (RXR) with TBT, the transcriptional levels of RXR and lipid metabolic genes, and the fatty acid content. The lethal concentration 10% (LC10) was determined to be 5.12 mu g/L TBT, and negative effects on ecologically relevant end points (e.g., decreased lifespan and fecundity) were detected at 5 mu g/L TBT. On the basis of these findings, subsequent experiments were conducted below 1 ug/L TBT, which did not show any negative effects on ecologically relevant end points in B. koreanus. Nile red staining analysis showed that after exposure to 1 mu g/L TBT, B. koreanus stored neutral lipids and had significantly increased transcriptional levels of RXR and lipid metabolism-related genes compared to the control. However, the content of total fatty acids did not significantly change at any exposure level. In the single fatty acids profile, a significant increase in saturated fatty acids (SFAs) 14:0 and 20:0 was observed, but the contents of omega-3 and omega-6 fatty acids were significantly decreased. Also, a transactivation assay of TBT with RXR showed that TBT is an agonist of Bk-RXR with a similar fold-induction to the positive control. Taken together, these results demonstrate that TBT-modulated RXR signaling leads to increase in transcriptional levels of lipid metabolism-related genes and the synthesis of SFAs but decreases the content of polyunsaturated fatty acids (PUFAs). Our findings support a wider taxonomic scope of lipid perturbation due to xenobiotic exposure that occurs via NRs in aquatic animals. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | American Chemical Society | - |
dc.title | Tributyltin Affects Retinoid X Receptor-Mediated Lipid Metabolism in the Marine Rotifer Brachionus koreanus | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Shin, Kyung-Hoon | - |
dc.identifier.doi | 10.1021/acs.est.9b01359 | - |
dc.identifier.scopusid | 2-s2.0-85069264643 | - |
dc.identifier.wosid | 000474478300065 | - |
dc.identifier.bibliographicCitation | Environmental Science & Technology, v.53, no.13, pp.7830 - 7839 | - |
dc.relation.isPartOf | Environmental Science & Technology | - |
dc.citation.title | Environmental Science & Technology | - |
dc.citation.volume | 53 | - |
dc.citation.number | 13 | - |
dc.citation.startPage | 7830 | - |
dc.citation.endPage | 7839 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Engineering | - |
dc.relation.journalResearchArea | Environmental Sciences & Ecology | - |
dc.relation.journalWebOfScienceCategory | Engineering, Environmental | - |
dc.relation.journalWebOfScienceCategory | Environmental Sciences | - |
dc.subject.keywordPlus | PERSISTENT ORGANIC POLLUTANTS | - |
dc.subject.keywordPlus | ADIPOCYTE DIFFERENTIATION | - |
dc.subject.keywordPlus | PEROXISOME PROLIFERATOR | - |
dc.subject.keywordPlus | ENDOCRINE DISRUPTION | - |
dc.subject.keywordPlus | ORGANOTIN COMPOUNDS | - |
dc.subject.keywordPlus | MONOGONONT ROTIFER | - |
dc.subject.keywordPlus | ENVIRONMENTAL LEVELS | - |
dc.subject.keywordPlus | CALORIC RESTRICTION | - |
dc.subject.keywordPlus | SIGNALING-PATHWAY | - |
dc.subject.keywordPlus | ADIPOSE-TISSUE | - |
dc.identifier.url | https://pubs.acs.org/doi/10.1021/acs.est.9b01359 | - |
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