Reversal of Hyperglycemia by Insulin-Secreting Rat Bone Marrow- and Blastocyst-Derived Hypoblast Stem Cell-Like Cells
DC Field | Value | Language |
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dc.contributor.author | Kumar, Anujith | - |
dc.contributor.author | Lo Nigro, Antonio | - |
dc.contributor.author | Gysemans, Conny | - |
dc.contributor.author | Cai, Qing | - |
dc.contributor.author | Esguerra, Camila | - |
dc.contributor.author | Nelson-Holte, Molly | - |
dc.contributor.author | Heremans, Yves | - |
dc.contributor.author | Jimenez-Gonzalez, Maria | - |
dc.contributor.author | Porciuncula, Angelo | - |
dc.contributor.author | Mathieu, Chantal | - |
dc.contributor.author | Binas, Bert | - |
dc.contributor.author | Heimberg, Harry | - |
dc.contributor.author | Prosper, Felipe | - |
dc.contributor.author | Hering, Bernhard | - |
dc.contributor.author | Verfaillie, Catherine M. | - |
dc.contributor.author | Barajas, Miguel | - |
dc.date.accessioned | 2021-06-23T03:23:27Z | - |
dc.date.available | 2021-06-23T03:23:27Z | - |
dc.date.issued | 2013-05 | - |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/28011 | - |
dc.description.abstract | beta-cell replacement may efficiently cure type 1 diabetic (T1D) patients whose insulin-secreting beta-cells have been selectively destroyed by autoantigen-reactive T cells. To generate insulin-secreting cells we used two cell sources: rat multipotent adult progenitor cells (rMAPC) and the highly similar rat extra-embryonic endoderm precursor (rXEN-P) cells isolated under rMAPC conditions from blastocysts (rHypoSC). rMAPC/rHypoSC were sequentially committed to definitive endoderm, pancreatic endoderm, and beta-cell like cells. On day 21, 20% of rMAPC/rHypoSC progeny expressed Pdx1 and C-peptide. rMAPCr/HypoSC progeny secreted C-peptide under the stimulus of insulin agonist carbachol, and was inhibited by the L-type voltage-dependent calcium channel blocker nifedipine. When rMAPC or rHypoSC differentiated d21 progeny were grafted under the kidney capsule of streptozotocin-induced diabetic nude mice, hyperglycemia reversed after 4 weeks in 6/10 rMAPC-and 5/10 rHypoSC-transplanted mice. Hyperglycemia recurred within 24 hours of graft removal and the histological analysis of the retrieved grafts revealed presence of Pdx1-, Nkx6.1- and C-peptide-positive cells. The ability of both rMAPC and HypoSC to differentiate to functional beta-cell like cells may serve to gain insight into signals that govern beta-cell differentiation and aid in developing culture systems to commit other (pluripotent) stem cells to clinically useful beta-cells for cell therapy of T1D. | - |
dc.format.extent | 11 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Public Library of Science | - |
dc.title | Reversal of Hyperglycemia by Insulin-Secreting Rat Bone Marrow- and Blastocyst-Derived Hypoblast Stem Cell-Like Cells | - |
dc.type | Article | - |
dc.publisher.location | 미국 | - |
dc.identifier.doi | 10.1371/journal.pone.0063491 | - |
dc.identifier.scopusid | 2-s2.0-84877359939 | - |
dc.identifier.wosid | 000319737700052 | - |
dc.identifier.bibliographicCitation | PLoS ONE, v.8, no.5, pp 1 - 11 | - |
dc.citation.title | PLoS ONE | - |
dc.citation.volume | 8 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 1 | - |
dc.citation.endPage | 11 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
dc.subject.keywordPlus | PANCREATIC BETA-CELLS | - |
dc.subject.keywordPlus | PROGENITOR CELLS | - |
dc.subject.keywordPlus | MOUSE PANCREAS | - |
dc.subject.keywordPlus | IN-VIVO | - |
dc.subject.keywordPlus | DIFFERENTIATION | - |
dc.subject.keywordPlus | ENDODERM | - |
dc.subject.keywordPlus | GENE | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | CULTURE | - |
dc.subject.keywordPlus | LIVER | - |
dc.subject.keywordAuthor | PROGENITOR CELLS | - |
dc.subject.keywordAuthor | GENE | - |
dc.subject.keywordAuthor | MOUSE PANCREAS | - |
dc.subject.keywordAuthor | IN-VIVO | - |
dc.subject.keywordAuthor | LIVER | - |
dc.subject.keywordAuthor | PANCREATIC BETA-CELLS | - |
dc.subject.keywordAuthor | ENDODERM | - |
dc.subject.keywordAuthor | DIFFERENTIATION | - |
dc.subject.keywordAuthor | EXPRESSION | - |
dc.subject.keywordAuthor | CULTURE | - |
dc.identifier.url | https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0063491 | - |
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