Corrigendum to “Identification and characterization of wblA-dependent tmcT regulation during tautomycetin biosynthesis in Streptomyces sp. CK4412” [Biotechnol. Adv. 30 (2012) 202–209]
DC Field | Value | Language |
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dc.contributor.author | Nah, Ji-Hye | - |
dc.contributor.author | Park, Shin-Hae | - |
dc.contributor.author | Yoon, Hye-Mi | - |
dc.contributor.author | Choi, Si-Sun | - |
dc.contributor.author | Lee, Chul-Hoon | - |
dc.contributor.author | Kim, Eung-Soo | - |
dc.date.accessioned | 2021-06-23T06:04:54Z | - |
dc.date.available | 2021-06-23T06:04:54Z | - |
dc.date.issued | 2012-11 | - |
dc.identifier.issn | 0734-9750 | - |
dc.identifier.issn | 1873-1899 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/31371 | - |
dc.description.abstract | Tautomycetin (TMC) is an unusual linear polyketide compound esterified with a cyclic anhydride. It exhibitsnovel activated T cell-specific immunosuppressant as well as anti-cancer activities. Previously, we isolatedand characterized the entire TMC biosynthetic gene cluster fromStreptomycessp. CK4412, including a TMCpathway-specific gene,tmcN, the over-expression of which led to a significant increase in TMC productivity. Inaddition, we also reported that WblA acts as a global down-regulator of antibiotic biosynthesis throughpathway-specific regulation inStreptomycesspecies. Here, we confirm that TmcT acts as another TMCpathway-specific regulator within the TMC biosynthetic cluster. Specifically,tmcTdeletion resulted in thecomplete loss of TMC production, whereas complementation with atmcT-carrying integrative plasmidsignificantly restored TMC biosynthesis. We also identified a 0.39 kbwblAortholog (namedwblAtmc) fromStreptomycessp. CK4412viagenomic DNA library screening that showed 96% amino acid identity compared toa previously-knownS. coelicolor wblA. Targeted gene disruption ofwblAtmcinStreptomycessp. CK4412exhibited approximately 3-fold higher TMC productivity than that in the wild-type strain. Moreover,transcription analyses of the TMC biosynthetic and regulatory genes revealed that the expression oftmcTwasstrongly down-regulated bywblAtmc. These results imply that the TMC biosynthetic regulation network iscontrolled by two pathway-specific positive regulator, WblAtmc-dependent TmcT as well as WblAtmc-independent TmcN inStreptomycessp. CK4412. | - |
dc.format.extent | 1 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Elsevier BV | - |
dc.title | Corrigendum to “Identification and characterization of wblA-dependent tmcT regulation during tautomycetin biosynthesis in Streptomyces sp. CK4412” [Biotechnol. Adv. 30 (2012) 202–209] | - |
dc.type | Article | - |
dc.publisher.location | 영국 | - |
dc.identifier.doi | 10.1016/j.biotechadv.2012.02.011 | - |
dc.identifier.scopusid | 2-s2.0-84867725260 | - |
dc.identifier.wosid | 000311859100049 | - |
dc.identifier.bibliographicCitation | Biotechnology Advances, v.30, no.6, pp 1750 - 1750 | - |
dc.citation.title | Biotechnology Advances | - |
dc.citation.volume | 30 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 1750 | - |
dc.citation.endPage | 1750 | - |
dc.type.docType | Correction | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S0734975012000626?via%3Dihub#! | - |
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