Potential application of antibody-mimicking peptides identified by phage display in immuno-magnetic separation of an antigen
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Thai Bao Dieu Hien | - |
dc.contributor.author | Maeng, Joon-Ho | - |
dc.contributor.author | Lee, Byung Heon | - |
dc.contributor.author | Seong, Gi Hun | - |
dc.contributor.author | Choo, Jaebum | - |
dc.contributor.author | Lee, Eungyo | - |
dc.date.accessioned | 2021-06-23T06:24:53Z | - |
dc.date.available | 2021-06-23T06:24:53Z | - |
dc.date.issued | 2012-10 | - |
dc.identifier.issn | 0168-1656 | - |
dc.identifier.issn | 1873-4863 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/31761 | - |
dc.description.abstract | Phage display was performed against human IgG (hIgG) through five rounds of 'biopanning'. Each round consisted of: (1) incubating a library of phage-displayed 12-mer peptides sequences on hIgG-coated magnetic beads, (2) washing the unbound phages, and (3) eluting the bound phages. The eluted phages were either amplified to enrich the pool of positive clones or subjected to the next round without amplification. Through ELISA, four clones (F9, D1, G5, and A10) showing specific binding affinity to hIgG were identified. Among these, F9 had the highest affinity (K-d = 6.2 nM), only one order of magnitude lower than the native anti-hIgG antibody (0.66 nM). Following the DNA sequences of the selected clones, four 12-mer peptides were chemically synthesized. Among them, D1 peptide showed the highest binding affinity to hIgG via SPR biosensor measurements. This peptide was conjugated to biofunctionalized magnetic beads, and its immuno-binding ability was compared with that of the native antibody immobilized to magnetic beads. The mol-to-mol binding efficacy of the peptide-coated magnetic beads was approximately 1000-fold lower than that of the antibody-coated magnetic beads. Our results suggest a feasibility of using antibody-mimicking peptides identified by phage display technique for immuno-magnetic separation of an antigen. (C) 2012 Elsevier B.V. All rights reserved. | - |
dc.format.extent | 8 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.title | Potential application of antibody-mimicking peptides identified by phage display in immuno-magnetic separation of an antigen | - |
dc.type | Article | - |
dc.publisher.location | 네델란드 | - |
dc.identifier.doi | 10.1016/j.jbiotec.2012.06.039 | - |
dc.identifier.scopusid | 2-s2.0-84864540079 | - |
dc.identifier.wosid | 000308354100005 | - |
dc.identifier.bibliographicCitation | JOURNAL OF BIOTECHNOLOGY, v.161, no.3, pp 213 - 220 | - |
dc.citation.title | JOURNAL OF BIOTECHNOLOGY | - |
dc.citation.volume | 161 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 213 | - |
dc.citation.endPage | 220 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.subject.keywordPlus | HUMAN SERUM | - |
dc.subject.keywordPlus | LIBRARIES | - |
dc.subject.keywordPlus | IMMOBILIZATION | - |
dc.subject.keywordPlus | DEPLETION | - |
dc.subject.keywordPlus | PROTEOME | - |
dc.subject.keywordAuthor | Phage display | - |
dc.subject.keywordAuthor | Biopanning | - |
dc.subject.keywordAuthor | Magnetic bead separation | - |
dc.subject.keywordAuthor | Antibody mimicking peptides | - |
dc.subject.keywordAuthor | Human immunoglobulin | - |
dc.subject.keywordAuthor | Immuno-binding | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S016816561200377X?via%3Dihub | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
55 Hanyangdeahak-ro, Sangnok-gu, Ansan, Gyeonggi-do, 15588, Korea+82-31-400-4269 sweetbrain@hanyang.ac.kr
COPYRIGHT © 2021 HANYANG UNIVERSITY. ALL RIGHTS RESERVED.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.