Different GATA factors dictate CCR3 transcription in a cell type-specific manner of allergic inflammatory cells
DC Field | Value | Language |
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dc.contributor.author | Chung, Il Yup | - |
dc.contributor.author | Kong, Su-Kang | - |
dc.contributor.author | Kim, Byung Soo | - |
dc.contributor.author | Uhm, Tae Gi | - |
dc.contributor.author | Hwang, Sae Mi | - |
dc.contributor.author | Kang, Jin Hyun | - |
dc.date.accessioned | 2021-06-23T07:40:08Z | - |
dc.date.available | 2021-06-23T07:40:08Z | - |
dc.date.created | 2021-01-21 | - |
dc.date.issued | 2012-05 | - |
dc.identifier.issn | 0022-1767 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/33011 | - |
dc.description.abstract | CCR3 is expressed in prominent allergic inflammatory cells, including eosinophils, mast cells, and Th2 cells, which preferentially express GATA-1, GATA-2, and GATA-3, respectively. We have previously demonstrated GATA-1-mediated CCR3 transcription with functional mapping of a GATA element in the regulatory region of CCR3 gene. Herein, we investigated whether GATA factors other than GATA-1 play a major role in CCR3 transcription in these cell types. A CCR3 reporter plasmid, but not a mutant CCR3 reporter lacking the functional GATA element, was activated in all three cell types, EoL-1 eosinophilic cells, HMC-1 master cells, and Jurkat T cells, suggesting a critical involvement of the GATA element regardless of cell types expressing different GATA factors. Knockdown assay showed that siRNAs directed to GATA-1 and GATA-2 significantly reduced CCR3 transactivation with an additive effect in EoL-1 and HMC-1 cells, while GATA-3 siRNA exclusively abrogated CCR3 transcription in Jurkat cells. In parallel, EMSA analysis showed that the functional GATA element preferentially bound GATA-1, GATA-2, and GATA-3 with nuclear extracts from EoL-1, HMC-1, and Jurkat T cells, respectively. These results highlight that different GATA factors are engaged in CCR3 transcription in a cell type-specific fashion, depending on the major allergic inflammatory cells. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | AMER ASSOC IMMUNOLOGISTS | - |
dc.title | Different GATA factors dictate CCR3 transcription in a cell type-specific manner of allergic inflammatory cells | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Chung, Il Yup | - |
dc.identifier.wosid | 000304659700122 | - |
dc.identifier.bibliographicCitation | JOURNAL OF IMMUNOLOGY, v.188 | - |
dc.relation.isPartOf | JOURNAL OF IMMUNOLOGY | - |
dc.citation.title | JOURNAL OF IMMUNOLOGY | - |
dc.citation.volume | 188 | - |
dc.type.rims | ART | - |
dc.type.docType | Meeting Abstract | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Immunology | - |
dc.relation.journalWebOfScienceCategory | Immunology | - |
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