Rapid identification and evaluation of antioxidant compounds from extracts of Petasites japonicus by hyphenated-HPLC techniques
- Authors
- Kim, Sang Min; Kang, Suk Woo; Jeon, Je-Seung; Jung, Yu-Jin; Kim, Chul Young; Pan, Cheol Ho; Um, Byung-Hun
- Issue Date
- Feb-2012
- Publisher
- WILEY
- Keywords
- hyphenated techniques; LC; UV-MS; LC-NMR; on-line antioxidant activity; Petasites japonicus; radical scavenging activity
- Citation
- BIOMEDICAL CHROMATOGRAPHY, v.26, no.2, pp.199 - 207
- Indexed
- SCIE
SCOPUS
- Journal Title
- BIOMEDICAL CHROMATOGRAPHY
- Volume
- 26
- Number
- 2
- Start Page
- 199
- End Page
- 207
- URI
- https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/33866
- DOI
- 10.1002/bmc.1646
- ISSN
- 0269-3879
- Abstract
- Gradient HPLC coupled to Diode Array Detector (DAD), MS/MS and NMR was applied to the rapid structure determination of major compounds of methanol extracts from leaves and roots of Petasites japonicus. The relative antioxidant capacities of the compounds were evaluated by an HPLC system with post-column on-line antioxidant detection based on 2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonic acid radical scavenging. Six compounds were successfully separated on a reverse-phase C18 column and were identified as 5-caffeoylquinic acid (5-CQA), fukinolic acid (FA), 3,5-di-O-caffeoylquinic acid (3,5-DCQA), quercetin-3-O-(6-acetyl)-beta-glucopyranoside (QAG), 4,5-di-O-caffeoylquinic acid (4,5-DCQA) and kaempferol-3-O-(6-acetyl)-beta-glucopyranoside (KAG) by MS/MS and 1H NMR data. Among these compounds, those containing a caffeoyl moiety (5-CQA, FA, 3,5- and 4,5-DCQA) showed relatively strong radical scavenging capacity, with 3,5-DCQA having the greatest radical scavenging capacity in leaf (23.09% of total antioxidant capacity) and root (26.47%) extracts. The relative radical scavenging portion of QAG was only 3.41% in the leaves and KAG did not show any radical scavenging activity. These results demonstrate that the hyphenated HPLC techniques can be successfully applied to rapidly identify structures and evaluate antioxidant activities without prior purification of compounds from plant tissues of P. japonicus. Copyright (C) 2011 John Wiley & Sons, Ltd.
- Files in This Item
-
Go to Link
- Appears in
Collections - COLLEGE OF PHARMACY > DEPARTMENT OF PHARMACY > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/33866)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.