Identification and characterization of wblA-dependent tmcT regulation during tautomycetin biosynthesis in Streptomyces sp CK4412
DC Field | Value | Language |
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dc.contributor.author | Nah, Ji-Hye | - |
dc.contributor.author | Park, Shin-Hae | - |
dc.contributor.author | Yoon, Hye-Mi | - |
dc.contributor.author | Choi, Si-Sun | - |
dc.contributor.author | Lee, Chul-Hoon | - |
dc.contributor.author | Kim, Eung-Soo | - |
dc.date.accessioned | 2021-06-23T08:07:32Z | - |
dc.date.available | 2021-06-23T08:07:32Z | - |
dc.date.issued | 2012-01 | - |
dc.identifier.issn | 0734-9750 | - |
dc.identifier.issn | 1873-1899 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/33924 | - |
dc.description.abstract | Tautomycetin (TMC) is an unusual linear polyketide compound esterified with a cyclic anhydride. It exhibits novel activated T cell-specific immunosuppressant as well as anti-cancer activities. Previously, we isolated and characterized the entire TMC biosynthetic gene cluster from Streptomyces sp. CK4412, including a TMC pathway-specific gene, tmcN, the over-expression of which led to a significant increase in TMC productivity. In addition, we also reported that WblA acts as a global down-regulator of antibiotic biosynthesis through pathway-specific regulation in Streptomyces species. Here, we confirm that TmcT acts as another TMC pathway-specific regulator within the TMC biosynthetic cluster. Specifically, tmcT deletion resulted in the complete loss of TMC production, whereas complementation with a tmcT-carrying integrative plasmid significantly restored TMC biosynthesis. We also identified a 0.39 kb wblA ortholog (named wblA(tmc)) from Streptomyces sp. CK4412 via genomic DNA library screening that showed 96% amino acid identity compared to a previously-known S. coelicolor wblA. Targeted gene disruption of wblA(tmc) in Streptomyces sp. CK4412 exhibited approximately 3-fold higher TMC productivity than that in the wild-type strain. Moreover, transcription analyses of the TMC biosynthetic and regulatory genes revealed that the expression of tmcT was strongly down-regulated by wblA(tmc). These results imply that the TMC biosynthetic regulation network is controlled by two pathway-specific positive regulator, WblA(tmc)-dependent TmcT as well as WblA(tmc)-independent TmcN in Streptomyces sp. CK4412. (C) 2011 Elsevier Inc. All rights reserved. | - |
dc.format.extent | 8 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Elsevier BV | - |
dc.title | Identification and characterization of wblA-dependent tmcT regulation during tautomycetin biosynthesis in Streptomyces sp CK4412 | - |
dc.type | Article | - |
dc.publisher.location | 영국 | - |
dc.identifier.doi | 10.1016/j.biotechadv.2011.05.004 | - |
dc.identifier.scopusid | 2-s2.0-84855731978 | - |
dc.identifier.wosid | 000300479200014 | - |
dc.identifier.bibliographicCitation | Biotechnology Advances, v.30, no.1, pp 202 - 209 | - |
dc.citation.title | Biotechnology Advances | - |
dc.citation.volume | 30 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 202 | - |
dc.citation.endPage | 209 | - |
dc.type.docType | Review | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.subject.keywordPlus | DNA-BINDING PROTEIN | - |
dc.subject.keywordPlus | POSITIVE REGULATOR | - |
dc.subject.keywordPlus | CONJUGAL TRANSFER | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | GENE-CLUSTER | - |
dc.subject.keywordPlus | PATHWAY | - |
dc.subject.keywordPlus | COELICOLOR | - |
dc.subject.keywordPlus | INDUCTION | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordAuthor | Streptomyces | - |
dc.subject.keywordAuthor | Tautomycetin | - |
dc.subject.keywordAuthor | wblA | - |
dc.subject.keywordAuthor | tmcT | - |
dc.subject.keywordAuthor | Immunosuppressant | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S0734975011000590?via%3Dihub | - |
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