Simultaneous Quantitative Determination of Nine Hallucinogenic NBOMe Derivatives in Human Plasma Using Liquid Chromatography Tandem Mass Spectrometryopen access
- Authors
- Seo, Hyewon; Yoo, Hye Hyun; Kim, Young-Hoon; Hong, Jin; Sheen, Yhun Yhong
- Issue Date
- Mar-2019
- Publisher
- 사단법인 한국질량분석학회
- Keywords
- New psychoactive substances; simultaneous determination; LC-MS/MS; solid-phase extraction; human plasma
- Citation
- Mass Spectrometry Letters, v.10, no.1, pp.18 - 26
- Indexed
- SCOPUS
KCI
- Journal Title
- Mass Spectrometry Letters
- Volume
- 10
- Number
- 1
- Start Page
- 18
- End Page
- 26
- URI
- https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/3466
- DOI
- 10.5478/MSL.2019.10.1.18
- ISSN
- 2233-4203
- Abstract
- We developed a bioanalytical method for simultaneous determination of nine NBOMe derivatives (25H-NBOMe, 25B-NBOMe, 25E-NBOMe, 25N-NBOMe, 25C-NBOH, 25I-NBOH, 25B-NBF, 25C-NBF, and 25I-NBF) in human plasma using liquid chromatography tandem mass spectrometry (LC-MS/MS). Human plasma samples were pre-treated using solid-phase extraction. Separation was achieved on a C18 column under gradient elution using a mobile phase containing 0.1% formic acid in acetonitrile and 0.1% formic acid in water at a flow rate of 0.3 mL/min. Mass detection was performed in the positive ion mode using multiple reaction monitoring. The calibration range was 1-100 ng/mL for all quantitative analytes, with a correlation coefficient greater than 0.99. The intra-and inter-day precision and accuracy varied from 0.85 to 6.92% and from 90.19 to 108.69%, respectively. The recovery ranged from 86.36 to 118.52%, and the matrix effects ranged from 27.09 to 99.72%. The stability was acceptable in various conditions. The LC-MS/MS method was validated for linearity, accuracy, precision, matrix effects, recovery and stability in accordance with the FDA guidance. The proposed method is suitable for reliable and robust routine screening and analysis of nine NBOMe derivatives in forensic field.
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