7-O -Methylwogonin from Scutellaria baicalensis Disturbs Mitotic Progression by Inhibiting Plk1 Activity in Hep3B Cells
DC Field | Value | Language |
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dc.contributor.author | Woo, Sang-Uk | - |
dc.contributor.author | Jang, Hay-Ran | - |
dc.contributor.author | Chin, Young-Won | - |
dc.contributor.author | Yim, Hyungshin | - |
dc.date.accessioned | 2021-06-22T10:25:56Z | - |
dc.date.available | 2021-06-22T10:25:56Z | - |
dc.date.created | 2021-01-21 | - |
dc.date.issued | 2019-02 | - |
dc.identifier.issn | 0032-0943 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/3541 | - |
dc.description.abstract | Polo-like kinase 1, a mitotic Ser/Thr kinase, has emerged as a molecular target for the development of anticancer drugs. In this study, we found that polo-like kinase 1 activity was inhibited by 7- O -methylwogonin and related flavones, including baicalein, dihydrobaicalein, and viscidulin II, isolated from Scutellaria baicalensis . Although dihydrobaicalein exhibited the highest polo-like kinase 1 inhibitory activity among the four compounds, it also inhibited other kinases, such as vaccinia-related kinase 2 and polo-like kinase 2. Baicalein and viscidulin II also showed low selectivity to polo-like kinase 1 since they inhibited polo-like kinase 3 and polo-like kinase 2, respectively. However, 7- O -methylwogonin exhibited selective polo-like kinase 1 inhibitory activity, as evidenced from in vitro kinase assays based on fluorescence resonance energy transfer assays and ADP-Glo kinase assays. In addition, examination of mitotic morphology and immunostaining using specific antibodies for the mitotic markers, p-histone H3 and mitotic protein monoclonal 2, in Hep3B cells showed that 7- O -methylwogonin treatment increased mitotic cell populations due to inhibition of mitotic progression as a result of polo-like kinase 1 inhibition. The pattern of 7- O -methylwogonin-induced mitotic arrest was similar to that of BI 2536, a specific polo-like kinase 1 inhibitor. Thus, it was suggested that 7- O -methylwogonin disturbed mitotic progression by inhibiting polo-like kinase 1 activity. These data suggest that 7- O -methylwogonin, a polo-like kinase 1 inhibitor, may be a useful anticancer agent because of its polo-like kinase 1 selectivity and effectiveness. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | GEORG THIEME VERLAG KG | - |
dc.title | 7-O -Methylwogonin from Scutellaria baicalensis Disturbs Mitotic Progression by Inhibiting Plk1 Activity in Hep3B Cells | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Yim, Hyungshin | - |
dc.identifier.doi | 10.1055/a-0731-0394 | - |
dc.identifier.scopusid | 2-s2.0-85061136244 | - |
dc.identifier.wosid | 000459790700005 | - |
dc.identifier.bibliographicCitation | PLANTA MEDICA, v.85, no.3, pp.217 - 224 | - |
dc.relation.isPartOf | PLANTA MEDICA | - |
dc.citation.title | PLANTA MEDICA | - |
dc.citation.volume | 85 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 217 | - |
dc.citation.endPage | 224 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Plant Sciences | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalResearchArea | Integrative & Complementary Medicine | - |
dc.relation.journalWebOfScienceCategory | Plant Sciences | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Medicinal | - |
dc.relation.journalWebOfScienceCategory | Integrative & Complementary Medicine | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.subject.keywordPlus | POLO-LIKE-KINASE | - |
dc.subject.keywordPlus | PHASE-I | - |
dc.subject.keywordPlus | CANCER-CELLS | - |
dc.subject.keywordPlus | TUMOR-GROWTH | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordAuthor | 7-O-methylwogonin | - |
dc.subject.keywordAuthor | polo-like kinase 1 | - |
dc.subject.keywordAuthor | Scutellaria baicalensis | - |
dc.subject.keywordAuthor | Lamiaceae | - |
dc.subject.keywordAuthor | anticancer | - |
dc.subject.keywordAuthor | mitosis | - |
dc.identifier.url | https://www.thieme-connect.de/products/ejournals/abstract/10.1055/a-0731-0394 | - |
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