Asymmetric maltose neopentyl glycol amphiphiles for a membrane protein study: effect of detergent asymmetricity on protein stability
DC Field | Value | Language |
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dc.contributor.author | Bae, Hyoung Eun | - |
dc.contributor.author | Du, Yang | - |
dc.contributor.author | Hariharan, Parameswaran | - |
dc.contributor.author | Mortensen, Jonas S. | - |
dc.contributor.author | Kumar, Kaavya K. | - |
dc.contributor.author | Ha, Betty | - |
dc.contributor.author | Das, Manabendra | - |
dc.contributor.author | Lee, Hyun Sung | - |
dc.contributor.author | Loland, Claus J. | - |
dc.contributor.author | Guan, Lan | - |
dc.contributor.author | Kobilka, Brian K. | - |
dc.contributor.author | Chae, Pil Seok | - |
dc.date.accessioned | 2021-06-22T10:26:05Z | - |
dc.date.available | 2021-06-22T10:26:05Z | - |
dc.date.created | 2021-01-21 | - |
dc.date.issued | 2019-01 | - |
dc.identifier.issn | 2041-6520 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/3556 | - |
dc.description.abstract | Maintaining protein stability in an aqueous solution is a prerequisite for protein structural and functional studies, but conventional detergents have increasingly showed limited ability to maintain protein integrity. A representative novel agent, maltose neopentyl glycol-3 (MNG-3), has recently substantially contributed to membrane protein structural studies. Motivated by the popular use of this novel agent, we prepared asymmetric versions of MNG-3 and evaluated these agents with several membrane proteins including two G protein-coupled receptors in this study. We found that some new MNGs were significantly more effective than MNG-3 at preserving protein integrity in the long term, suggesting that these asymmetric MNGs will find a wide use in membrane protein studies. In addition, this is the first study addressing the favorable effect of detergent asymmetric nature on membrane protein stability. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | Royal Society of Chemistry | - |
dc.title | Asymmetric maltose neopentyl glycol amphiphiles for a membrane protein study: effect of detergent asymmetricity on protein stability | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Chae, Pil Seok | - |
dc.identifier.doi | 10.1039/c8sc02560f | - |
dc.identifier.scopusid | 2-s2.0-85060708991 | - |
dc.identifier.wosid | 000457342200015 | - |
dc.identifier.bibliographicCitation | Chemical Science, v.10, no.4, pp.1107 - 1116 | - |
dc.relation.isPartOf | Chemical Science | - |
dc.citation.title | Chemical Science | - |
dc.citation.volume | 10 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 1107 | - |
dc.citation.endPage | 1116 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.subject.keywordPlus | CRYSTAL-STRUCTURE | - |
dc.subject.keywordPlus | BETA(2)-ADRENERGIC RECEPTOR | - |
dc.subject.keywordPlus | ALLOSTERIC MODULATION | - |
dc.subject.keywordPlus | SOLUBILIZATION | - |
dc.subject.keywordPlus | STABILIZATION | - |
dc.subject.keywordPlus | INSIGHTS | - |
dc.subject.keywordPlus | BINDING | - |
dc.subject.keywordPlus | CRYSTALLIZATION | - |
dc.subject.keywordPlus | AMPHIPOLS | - |
dc.subject.keywordPlus | ANALOGS | - |
dc.identifier.url | https://pubs.rsc.org/en/content/articlelanding/2019/SC/C8SC02560F | - |
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