Sublingual Immunization with M2-Based Vaccine Induces Broad Protective Immunity against Influenza
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Shim, Byoung-Shik | - |
dc.contributor.author | Choi, Young Ki | - |
dc.contributor.author | Yun, Cheol-Heui | - |
dc.contributor.author | Lee, Eu-Gene | - |
dc.contributor.author | Jeon, Yoon Seong | - |
dc.contributor.author | Park, Sung-Moo | - |
dc.contributor.author | Cheon, In Su | - |
dc.contributor.author | Joo, Dong-Hyun | - |
dc.contributor.author | Cho, Chung Hwan | - |
dc.contributor.author | Song, Min-Suk | - |
dc.contributor.author | Seo, Sang-Uk | - |
dc.contributor.author | Byun, Young-Ho | - |
dc.contributor.author | Park, Hae-Jung | - |
dc.contributor.author | Poo, Haryoung | - |
dc.contributor.author | Seong, Baik Lin | - |
dc.contributor.author | Kim, Jae Ouk | - |
dc.contributor.author | Nguyen, Huan Huu | - |
dc.contributor.author | Stadler, Konrad | - |
dc.contributor.author | Kim, Dong Wook | - |
dc.contributor.author | Hong, Kee-Jong | - |
dc.contributor.author | Czerkinsky, Cecil | - |
dc.contributor.author | Song, Man Ki | - |
dc.date.accessioned | 2021-06-23T10:04:57Z | - |
dc.date.available | 2021-06-23T10:04:57Z | - |
dc.date.created | 2021-01-21 | - |
dc.date.issued | 2011-11 | - |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/36393 | - |
dc.description.abstract | Background: The ectodomain of matrix protein 2 (M2e) of influenza A virus is a rationale target antigen candidate for the development of a universal vaccine against influenza as M2e undergoes little sequence variation amongst human influenza A strains. Vaccine-induced M2e-specific antibodies (Abs) have been shown to display significant cross-protective activity in animal models. M2e-based vaccine constructs have been shown to be more protective when administered by the intranasal (i.n.) route than after parenteral injection. However, i.n. administration of vaccines poses rare but serious safety issues associated with retrograde passage of inhaled antigens and adjuvants through the olfactory epithelium. In this study, we examined whether the sublingual (s.l.) route could serve as a safe and effective alternative mucosal delivery route for administering a prototype M2e-based vaccine. The mechanism whereby s.l. immunization with M2e vaccine candidate induces broad protection against infection with different influenza virus subtypes was explored. Methods and Results: A recombinant M2 protein with three tandem copies of the M2e (3M2eC) was expressed in Escherichia coli. Parenteral immunizations of mice with 3M2eC induced high levels of M2e-specific serum Abs but failed to provide complete protection against lethal challenge with influenza virus. In contrast, s.l. immunization with 3M2eC was superior for inducing protection in mice. In the latter animals, protection was associated with specific Ab responses in the lungs. Conclusions: The results demonstrate that s.l. immunization with 3M2eC vaccine induced airway mucosal immune responses along with broad cross-protective immunity to influenza. These findings may contribute to the understanding of the M2-based vaccine approach to control epidemic and pandemic influenza infections. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | PUBLIC LIBRARY SCIENCE | - |
dc.title | Sublingual Immunization with M2-Based Vaccine Induces Broad Protective Immunity against Influenza | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Dong Wook | - |
dc.identifier.doi | 10.1371/journal.pone.0027953 | - |
dc.identifier.scopusid | 2-s2.0-82355170321 | - |
dc.identifier.wosid | 000298168100027 | - |
dc.identifier.bibliographicCitation | PLOS ONE, v.6, no.11, pp.1 - 10 | - |
dc.relation.isPartOf | PLOS ONE | - |
dc.citation.title | PLOS ONE | - |
dc.citation.volume | 6 | - |
dc.citation.number | 11 | - |
dc.citation.startPage | 1 | - |
dc.citation.endPage | 10 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
dc.subject.keywordPlus | INTEGRAL MEMBRANE-PROTEIN | - |
dc.subject.keywordPlus | ANTIGENIC PEPTIDE VACCINE | - |
dc.subject.keywordPlus | MONOCLONAL-ANTIBODY | - |
dc.subject.keywordPlus | H5N1 INFLUENZA | - |
dc.subject.keywordPlus | EXTRACELLULAR DOMAIN | - |
dc.subject.keywordPlus | VIRUS-INFECTION | - |
dc.subject.keywordPlus | M2 | - |
dc.subject.keywordPlus | MUCOSAL | - |
dc.subject.keywordPlus | MICE | - |
dc.subject.keywordPlus | ECTODOMAIN | - |
dc.subject.keywordAuthor | ANTIGENIC PEPTIDE VACCINE | - |
dc.subject.keywordAuthor | M2 PROTEIN | - |
dc.subject.keywordAuthor | MATRIX PROTEIN-2 | - |
dc.subject.keywordAuthor | M-GENE | - |
dc.subject.keywordAuthor | EXTRACELLULAR DOMAIN | - |
dc.subject.keywordAuthor | MONOCLONAL-ANTIBODY | - |
dc.subject.keywordAuthor | MICE | - |
dc.subject.keywordAuthor | A-VIRUS | - |
dc.subject.keywordAuthor | H5N1 INFLUENZA | - |
dc.subject.keywordAuthor | INTEGRAL MEMBRANE-PROTEIN | - |
dc.identifier.url | https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0027953 | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
55 Hanyangdeahak-ro, Sangnok-gu, Ansan, Gyeonggi-do, 15588, Korea+82-31-400-4269 sweetbrain@hanyang.ac.kr
COPYRIGHT © 2021 HANYANG UNIVERSITY. ALL RIGHTS RESERVED.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.